Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-20135 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- 4EBP1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- A suggested positive control is NIH-3T3 cell lysate. PA5-20135 can be used with blocking peptide PEP-0253.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of 3T3 cell lysate using a 4E-BP1 polyclonal antibody (Product # PA5-20135) at (A) 2.5, (B) 5 and (C) 10 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of 4E-BP1 in 3T3 cell lysate with 4EBP1 Polyclonal Antibody (Product # PA5-20135) at (A) 2.5, (B) 5 and (C) 10 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry of 4E-BP1 in 3T3 cells with 4EBP1 Polyclonal Antibody (Product # PA5-20135) at 2 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence of 4E-BP1 in 3T3 cells with 4EBP1 Polyclonal Antibody (Product # PA5-20135) at 20 µg/mL. Green: 4E-BP1 Blue: DAPI staining
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry staining of 3T3 cells using a 4E-BP1 polyclonal antibody (Product # PA5-20135) at a 2 µg/mL dilution.