PA5-20135

antibody from Invitrogen Antibodies

Targeting: EIF4EBP1 4E-BP1, PHAS-I

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-20135

Provider

Invitrogen Antibodies

Product name

4EBP1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

A suggested positive control is NIH-3T3 cell lysate.

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of 3T3 cell lysate using a 4E-BP1 polyclonal antibody (Product # PA5-20135) at (A) 2.5, (B) 5 and (C) 10 µg/mL.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of 4E-BP1 in 3T3 cell lysate with 4EBP1 Polyclonal Antibody (Product # PA5-20135) at (A) 2.5, (B) 5 and (C) 10 µg/mL.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry of 4E-BP1 in 3T3 cells with 4EBP1 Polyclonal Antibody (Product # PA5-20135) at 2 µg/mL.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence of 4E-BP1 in 3T3 cells with 4EBP1 Polyclonal Antibody (Product # PA5-20135) at 20 µg/mL. Green: 4E-BP1 Blue: DAPI staining

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry staining of 3T3 cells using a 4E-BP1 polyclonal antibody (Product # PA5-20135) at a 2 µg/mL dilution.