Explore
Validate
Learn
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-28947 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- 4EBP1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Recommended positive controls: HeLa, H1299, HCT116, NB4, HL60. Predicted reactivity: Mouse (90%), Rat (92%), Pig (90%), Bovine (92%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of EIF4EBP1 using 30 µg of A) H1299 and B) HCT116 lysate. Samples were loaded onto a 15% SDS-PAGE gel and probed with an EIF4EBP1 polyclonal antibody (Product # PA5-28947) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 4E-BP1 antibody detects 4E-BP1 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with 4E-BP1 antibody 4EBP1 Polyclonal Antibody (Product # PA5-28947) diluted at a dilution of 1:2,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded RT2 xenograft, using EIF4EBP1 (Product # PA5-28947) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of 4E-BP1 was performed in HCT116 whole cell extracts using 5 µg of 4EBP1 Polyclonal Antibody (Product # PA5-28947). Samples were transferred to a membrane and probed with 4EBP1 Polyclonal Antibody as a primary antibody and an HRP-conjugated anti-Rabbit IgG was used as a secondary antibody.
