PA5-30679

antibody from Invitrogen Antibodies

Targeting: EIF4EBP1 4E-BP1, PHAS-I

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-30679

Provider

Invitrogen Antibodies

Product name

4EBP1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Recommended positive controls: 293T, HeLa.

Concentration

1.09 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of EIF4EBP1 using 30 µg of 293T lysate. Samples were loaded onto a 15% SDS-PAGE gel and probed with an EIF4EBP1 polyclonal antibody (Product # PA5-30679) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of 4E-BP1 in Various whole cell extracts (30 µg). Samples were separated by 15% SDS-PAGE and the membrane was probed with 4E-BP1 Polyclonal antibody (Product # PA5-30679) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

4E-BP1 antibody detects 4E-BP1 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with 4E-BP1 antibody 4EBP1 Polyclonal Antibody (Product # PA5-30679) diluted at a dilution of 1:1,000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

4EBP1 Polyclonal Antibody detects 4E-BP1 protein at cytoplasm by immunofluorescent analysis. Sample: HCT116 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: 4E-BP1 protein stained by 4EBP1 Polyclonal Antibody (Product # PA5-30679) diluted at 1:1,000. Blue: Hoechst 33342 staining. Scale bar = 10 µm.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

4EBP1 Polyclonal Antibody detects 4E-BP1 protein at cytoplasm by immunofluorescent analysis. Sample: HCT116 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: 4E-BP1 protein stained by 4EBP1 Polyclonal Antibody (Product # PA5-30679) diluted at 1:1,000. Blue: Hoechst 33342 staining. Scale bar = 10 µm.