60246-1-Ig

antibody from Proteintech Group

Targeting: EIF4EBP1 4E-BP1, PHAS-I

Provider product page for 60246-1-Ig

Western blot

ELISA

Immunocytochemistry

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [55]
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Validations
Western blot [1]
Immunohistochemistry [1]

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Product number: 60246-1-Ig - Provider product page
Provider: Proteintech Group
Product name: 4EBP1 antibody
Antibody type: Monoclonal
Description: KD/KO validated 4EBP1 antibody (Cat. #60246-1-Ig) is a mouse monoclonal antibody that shows reactivity with human, rat, pig, mouse and has been validated for the following applications: IF, WB,ELISA.
Reactivity: Human, Mouse, Rat, Porcine
Host: Mouse
Conjugate: Unconjugated
Isotype: IgG
Antibody clone number: 2C3F3
Vial size: 20ul, 150ul

EIF4EBP1 protein structure - 60246-1-Ig shown in red.

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Tumor Necrosis Factor Receptor-2 Signals Clear-Cell Renal Carcinoma Proliferation via Phosphorylated 4E Binding Protein-1 and Mitochondrial Gene Translation.

Al-Lamki RS, Tolkovsky AM, Alawwami M, Lu W, Field SF, Wang J, Pober JS, Bradley JR
The American journal of pathology 2024 Jul;194(7):1374-1387

CYP3A5 unexpectedly regulates glucose metabolism through the AKT-TXNIP-GLUT1 axis in pancreatic cancer.

Shao M, Pan Q, Tan H, Wu J, Lee HW, Huber AD, Wright WC, Cho JH, Yu J, Peng J, Chen T
Genes & diseases 2024 Jul;11(4):101079

Muscle fibre transition and transcriptional changes of horse skeletal muscles during traditional Mongolian endurance training.

Bou T, Ding W, Ren X, Liu H, Gong W, Jia Z, Zhang X, Dugarjaviin M, Bai D
Equine veterinary journal 2024 Jan;56(1):178-192

Stress granules affect the dual PI3K/mTOR inhibitor response by regulating the mitochondrial unfolded protein response.

Lin N, Sun L, Chai J, Qi H, Zhao Y, Ma J, Xia M, Hu X
Cancer cell international 2024 Jan 18;24(1):38

Protein phosphatase 1 regulatory subunit 15 A promotes translation initiation and induces G2M phase arrest during cuproptosis in cancers.

Liu C, Chen L, Cong Y, Cheng L, Shuai Y, Lv F, Chen K, Song Y, Xing Y
Cell death & disease 2024 Feb 16;15(2):149

miR-1304 targets KLK11 to regulate gastric cancer cell proliferation through the mTOR signaling pathway.

Ding Y, Wang Z, Chen C, Li D, Wang W, Jia Y, Qin Y
Carcinogenesis 2024 Feb 12;45(1-2):45-56

ATF6 supports lysosomal function in tumor cells to enable ER stress-activated macroautophagy and CMA: impact on mutant TP53 expression.

Benedetti R, Romeo MA, Arena A, Gilardini Montani MS, D'Orazi G, Cirone M
Autophagy 2024 Aug;20(8):1854-1867

Huayu Qutan Recipe promotes lipophagy and cholesterol efflux through the mTORC1/TFEB/ABCA1-SCARB1 signal axis.

Li Y, Pan J, Yu JJJ, Wu X, Yang G, Pan X, Sui G, Wang M, Cheng M, Zhu S, Tai H, Xiao H, Xu L, Wu J, Yang Y, Tang J, Gong L, Jia L, Min D
Journal of cellular and molecular medicine 2024 Apr;28(8):e18257

Weight bearing training alleviates muscle atrophy and pyroptosis of middle-aged rats.

Fu P, Gong L, Yang L, Tang S, Ma F
Frontiers in endocrinology 2023;14:1202686

Lysine methylation of PPP1CA by the methyltransferase SUV39H2 disrupts TFEB-dependent autophagy and promotes intervertebral disc degeneration.

Liang H, Luo R, Li G, Zhang W, Zhu D, Wu D, Zhou X, Tong B, Wang B, Feng X, Wang K, Song Y, Yang C
Cell death and differentiation 2023 Sep;30(9):2135-2150

Hirudin inhibits glioma growth through mTOR-regulated autophagy.

Ma Y, Wu S, Zhao F, Li H, Li Q, Zhang J, Li H, Yuan Z
Journal of cellular and molecular medicine 2023 Sep;27(18):2701-2713

UCHL1-PKM2 axis dysregulation is associated with promoted proliferation and invasiveness of urothelial bladder cancer cells.

Zheng Y, Shi D, Chen L, Yang Y, Yao M
Aging 2023 Oct 9;15(19):10593-10606

Toxicity mechanism of peri-implantation pesticide beta-cypermethrin exposure on endometrial remodeling in early pregnant mice.

Zhou YJ, Qiao QF, Wang LQ, Sheng TY, Cui MX, Chen QD, Wang CY, Zhang YX
Toxicology 2023 May 1;489:153497

JQ-1/bortezomib combination strongly impairs MM and PEL survival by inhibiting c-Myc and mTOR despite the activation of prosurvival mechanisms.

Arena A, Romeo MA, Benedetti R, Gilardini Montani MS, Cirone M
Experimental hematology 2023 Mar-Apr;119-120:28-41

Interruption of p53-MDM2 Interaction by Nutlin-3a in Human Lymphoma Cell Models Initiates a Cell-Dependent Global Effect on Transcriptome and Proteome Level.

Psatha K, Kollipara L, Drakos E, Deligianni E, Brintakis K, Patsouris E, Sickmann A, Rassidakis GZ, Aivaliotis M
Cancers 2023 Jul 31;15(15)

Chloride intracellular channel 1 promotes esophageal squamous cell carcinoma proliferation via mTOR signalling.

Geng H, Feng C, Sun Z, Fan X, Xie Y, Gu J, Fan L, Liu G, Li C, Thorne RF, Zhang XD, Li X, Liu X
Translational oncology 2023 Jan;27:101560

STRA6 Promotes Thyroid Carcinoma Progression via Activation of the ILK/AKT/mTOR Axis in Cells and Female Nude Mice.

He W, Cheng Z, Huo Z, Lin B, Wang X, Sun Y, Yu S, Cao S, Xue J, Liu R, Lv W, Li Y, Hong S, Xiao H
Endocrinology 2023 Jan 9;164(3)

The Key Role of Initiation Timing on Stroke Rehabilitation by Remote Ischemic Conditioning with Exercise (RICE).

Wang Q, Wehbe A, Wills M, Li F, Geng X, Ding Y
Neurological research 2023 Apr;45(4):334-345

Zheng Y, Qi B, Gao W, Qi Z, Liu Y, Wang Y, Feng J, Cheng X, Luo Z, Li T
Frontiers in cardiovascular medicine 2022;9:890321

Comparison of the Effects of Inorganic or Amino Acid-Chelated Zinc on Mouse Myoblast Growth in vitro and Growth Performance and Carcass Traits in Growing-Finishing Pigs.

Zhang L, Guo Q, Duan Y, Lin X, Ni H, Zhou C, Li F
Frontiers in nutrition 2022;9:857393

Asymptomatic Hyperuricemia Is Associated with Achilles Tendon Rupture through Disrupting the Normal Functions of Tendon Stem/Progenitor Cells.

Liang J, Chen B, Li Y, Nie D, Lei C, Yang Q, Zhou Y, Li S, Chen S, Li B, Shu L, Chen L, Wang W
Stem cells international 2022;2022:6795573

3,4-Dihydroxyphenylethanol (DPE or Hydroxytyrosol) Counteracts ERK1/2 and mTOR Activation, Pro-Inflammatory Cytokine Release, Autophagy and Mitophagy Reduction Mediated by Benzo[a]pyrene in Primary Human Colonic Epithelial Cells.

Santarelli R, Pompili C, Gilardini Montani MS, Evangelista L, Gonnella R, Cirone M
Pharmaceutics 2022 Mar 17;14(3)

ATF6 prevents DNA damage and cell death in colon cancer cells undergoing ER stress.

Benedetti R, Romeo MA, Arena A, Gilardini Montani MS, Di Renzo L, D'Orazi G, Cirone M
Cell death discovery 2022 Jun 25;8(1):295

Intracellular accumulation of tau inhibits autophagosome formation by activating TIA1-amino acid-mTORC1 signaling.

Li MZ, Liu EJ, Zhou QZ, Li SH, Liu SJ, Yu HT, Pan QH, Sun F, He T, Wang WJ, Ke D, Feng YQ, Li J, Wang JZ
Military Medical Research 2022 Jul 7;9(1):38

KAT7-mediated CANX (calnexin) crotonylation regulates leucine-stimulated MTORC1 activity.

Yan G, Li X, Zheng Z, Gao W, Chen C, Wang X, Cheng Z, Yu J, Zou G, Farooq MZ, Zhu X, Zhu W, Zhong Q, Yan X
Autophagy 2022 Dec;18(12):2799-2816

RIOK3-Mediated Akt phosphorylation facilitates synergistic replication of Marek's disease and reticuloendotheliosis viruses.

Du X, Zhou D, Zhou J, Xue J, Cheng Z
Virulence 2022 Dec;13(1):1184-1198

mTORC1 Mediates the Processes of Lysine Regulating Satellite Cells Proliferation, Apoptosis, and Autophagy.

Liu M, Yue Z, Zhang B, Li F, Liu L, Li F
Metabolites 2022 Aug 25;12(9)

PPT1 Reduction Contributes to Erianin-Induced Growth Inhibition in Oral Squamous Carcinoma Cells.

Luo Q, Li X, Gan G, Yang M, Chen X, Chen F
Frontiers in cell and developmental biology 2021;9:764263

Tetrandrine Modulates Rheb-mTOR Signaling-Mediated Selective Autophagy and Protects Pulmonary Fibrosis.

Liu Y, Zhong W, Zhang J, Chen W, Lu Y, Qiao Y, Zeng Z, Huang H, Cai S, Dong H
Frontiers in pharmacology 2021;12:739220

Therapeutic Potential of CUDC-907 (Fimepinostat) for Hepatocarcinoma Treatment Revealed by Tumor Spheroids-Based Drug Screening.

Liao W, Yang W, Xu J, Yan Z, Pan M, Xu X, Zhou S, Zhu Y, Lan J, Zeng M, Han X, Li S, Li Y, Liang K, Gao Y, Peng Q
Frontiers in pharmacology 2021;12:658197

UPF1 impacts on mTOR signaling pathway and autophagy in endometrioid endometrial carcinoma.

Zhang M, Chen H, Qin P, Cai T, Li L, Chen R, Liu S, Chen H, Lin W, Chen H, Strickland AL, Xiong H, Jiang Q
Aging 2021 Sep 14;13(17):21202-21215

TSPAN1 promotes autophagy flux and mediates cooperation between WNT-CTNNB1 signaling and autophagy via the MIR454-FAM83A-TSPAN1 axis in pancreatic cancer.

Zhou C, Liang Y, Zhou L, Yan Y, Liu N, Zhang R, Huang Y, Wang M, Tang Y, Ali DW, Wang Y, Michalak M, Chen XZ, Tang J
Autophagy 2021 Oct;17(10):3175-3195

New Insights into Curcumin- and Resveratrol-Mediated Anti-Cancer Effects.

Arena A, Romeo MA, Benedetti R, Masuelli L, Bei R, Gilardini Montani MS, Cirone M
Pharmaceuticals (Basel, Switzerland) 2021 Oct 22;14(11)

p53-R273H Sustains ROS, Pro-Inflammatory Cytokine Release and mTOR Activation While Reducing Autophagy, Mitophagy and UCP2 Expression, Effects Prevented by wtp53.

Romeo MA, Gilardini Montani MS, Benedetti R, Arena A, D'Orazi G, Cirone M
Biomolecules 2021 Feb 24;11(3)

Mammalian target of rapamycin signaling pathway is involved in synaptic plasticity of the spinal dorsal horn and neuropathic pain in rats by regulating autophagy.

Hu J, Chen X, Cheng J, Kong F, Xia H, Wu J
Neuroreport 2021 Aug 4;32(11):925-935

STYK1 promotes autophagy through enhancing the assembly of autophagy-specific class III phosphatidylinositol 3-kinase complex I.

Zhou C, Qian X, Hu M, Zhang R, Liu N, Huang Y, Yang J, Zhang J, Bai H, Yang Y, Wang Y, Ali D, Michalak M, Chen XZ, Tang J
Autophagy 2020 Oct;16(10):1786-1806

The circular RNA circ-ERBIN promotes growth and metastasis of colorectal cancer by miR-125a-5p and miR-138-5p/4EBP-1 mediated cap-independent HIF-1α translation.

Chen LY, Wang L, Ren YX, Pang Z, Liu Y, Sun XD, Tu J, Zhi Z, Qin Y, Sun LN, Li JM
Molecular cancer 2020 Nov 23;19(1):164

OGDHL silencing promotes hepatocellular carcinoma by reprogramming glutamine metabolism.

Dai W, Xu L, Yu X, Zhang G, Guo H, Liu H, Song G, Weng S, Dong L, Zhu J, Liu T, Guo C, Shen X
Journal of hepatology 2020 May;72(5):909-923

miR-196b-5p controls adipocyte differentiation and lipogenesis through regulating mTORC1 and TGF-β signaling.

Shi Y, Li F, Wang S, Wang C, Xie Y, Zhou J, Li X, Wang B
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2020 Jul;34(7):9207-9222

Feeding induces cholesterol biosynthesis via the mTORC1-USP20-HMGCR axis.

Lu XY, Shi XJ, Hu A, Wang JQ, Ding Y, Jiang W, Sun M, Zhao X, Luo J, Qi W, Song BL
Nature 2020 Dec;588(7838):479-484

Functional characterization of DLK1/MEG3 locus on chromosome 14q32.2 reveals the differentiation of pituitary neuroendocrine tumors.

Chen Y, Gao H, Liu Q, Xie W, Li B, Cheng S, Guo J, Fang Q, Zhu H, Wang Z, Wang J, Li C, Zhang Y
Aging 2020 Dec 29;13(1):1422-1439

A CRISPR knockout negative screen reveals synergy between CDKs inhibitor and metformin in the treatment of human cancer in vitro and in vivo.

Ma Y, Zhu Q, Liang J, Li Y, Li M, Zhang Y, Wang X, Zeng Y, Jiao Y
Signal transduction and targeted therapy 2020 Aug 19;5(1):152

Leucine attenuates muscle atrophy and autophagosome formation by activating PI3K/AKT/mTOR signaling pathway in rotator cuff tears.

Zheng R, Huang S, Zhu J, Lin W, Xu H, Zheng X
Cell and tissue research 2019 Oct;378(1):113-125

Integral membrane protein 2A inhibits cell growth in human breast cancer via enhancing autophagy induction.

Zhou C, Wang M, Yang J, Xiong H, Wang Y, Tang J
Cell communication and signaling : CCS 2019 Aug 22;17(1):105

FGFR2 Promotes Gastric Cancer Progression by Inhibiting the Expression of Thrombospondin4 via PI3K-Akt-Mtor Pathway.

Huang T, Liu D, Wang Y, Li P, Sun L, Xiong H, Dai Y, Zou M, Yuan X, Qiu H
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2018;50(4):1332-1345

Rapamycin Reduces Podocyte Apoptosis and is Involved in Autophagy and mTOR/ P70S6K/4EBP1 Signaling.

Jin J, Hu K, Ye M, Wu D, He Q
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2018;48(2):765-772

Cysteine-rich protein 61 regulates adipocyte differentiation from mesenchymal stem cells through mammalian target of rapamycin complex 1 and canonical Wnt signaling.

Yang Y, Qi Q, Wang Y, Shi Y, Yang W, Cen Y, Zhu E, Li X, Chen D, Wang B
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2018 Jun;32(6):3096-3107

Macrophages confer resistance to PI3K inhibitor GDC-0941 in breast cancer through the activation of NF-κB signaling.

Usman MW, Gao J, Zheng T, Rui C, Li T, Bian X, Cheng H, Liu P, Luo F
Cell death & disease 2018 Jul 24;9(8):809

EPS8 regulates proliferation, apoptosis and chemosensitivity in BCR-ABL positive cells via the BCR-ABL/PI3K/AKT/mTOR pathway.

Huang R, Liu H, Chen Y, He Y, Kang Q, Tu S, He Y, Zhou X, Wang L, Yang J, Wu A, Li Y
Oncology reports 2018 Jan;39(1):119-128

Highly Selective, Potent, and Oral mTOR Inhibitor for Treatment of Cancer as Autophagy Inducer.

Guo Q, Yu C, Zhang C, Li Y, Wang T, Huang Z, Wang X, Zhou W, Li Y, Qin Z, Wang C, Gao R, Nie Y, Ma Y, Shi Y, Zheng J, Yang S, Fan Y, Xiang R
Journal of medicinal chemistry 2018 Feb 8;61(3):881-904

MAN1B1 is associated with poor prognosis and modulates proliferation and apoptosis in bladder cancer.

Wang HF, Wu JH, Gai JW, Yang SQ, Ma QT, Ma HS, Feng Q
Gene 2018 Dec 30;679:314-319

Cucurbitacin B induces autophagy and apoptosis by suppressing CIP2A/PP2A/mTORC1 signaling axis in human cisplatin resistant gastric cancer cells.

Liu X, Duan C, Ji J, Zhang T, Yuan X, Zhang Y, Ma W, Yang J, Yang L, Jiang Z, Yu H, Liu Y
Oncology reports 2017 Jul;38(1):271-278

Effective use of PI3K inhibitor BKM120 and PARP inhibitor Olaparib to treat PIK3CA mutant ovarian cancer.

Wang D, Wang M, Jiang N, Zhang Y, Bian X, Wang X, Roberts TM, Zhao JJ, Liu P, Cheng H
Oncotarget 2016 Mar 15;7(11):13153-66

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: K-562 cells were subjected to SDS PAGE followed by western blot with 60246-1-Ig(4EBP1 antibody) at dilution of 1:2000
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: The 4EBP1 antibody from Proteintech is a mouse monoclonal antibody to a fusion protein of human 4EBP1. This antibody recognizes human,mouse antigen. The 4EBP1 antibody has been validated for the following applications: ELISA, WB, IHC analysis.
Appendix: 60246-1-Ig.pdf

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