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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [6]
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- Validations
- Western blot [1]
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- Product number
- ABIN307094 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1) antibody
- Antigen
- Other
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 10 Miniblots
- Concentration
- 1 mg/ml
- Storage
- Product should be stored at -20C. Aliquot to avoid freeze/thaw cycles
Submitted references Phosphoinositide 3-kinases as drug targets in cancer.
4E-binding protein phosphorylation and eukaryotic initiation factor-4E release are required for airway smooth muscle hypertrophy.
Strain-induced vascular endothelial cell proliferation requires PI3K-dependent mTOR-4E-BP1 signal pathway.
Distinct signaling events downstream of mTOR cooperate to mediate the effects of amino acids and insulin on initiation factor 4E-binding proteins.
Translational control of cell fate: availability of phosphorylation sites on translational repressor 4E-BP1 governs its proapoptotic potency.
Regulation of 4E-BP1 phosphorylation: a novel two-step mechanism.
Stephens L, Williams R, Hawkins P
Current opinion in pharmacology 2005 Aug;5(4):357-65
Current opinion in pharmacology 2005 Aug;5(4):357-65
4E-binding protein phosphorylation and eukaryotic initiation factor-4E release are required for airway smooth muscle hypertrophy.
Zhou L, Goldsmith AM, Bentley JK, Jia Y, Rodriguez ML, Abe MK, Fingar DC, Hershenson MB
American journal of respiratory cell and molecular biology 2005 Aug;33(2):195-202
American journal of respiratory cell and molecular biology 2005 Aug;33(2):195-202
Strain-induced vascular endothelial cell proliferation requires PI3K-dependent mTOR-4E-BP1 signal pathway.
Li W, Sumpio BE
American journal of physiology. Heart and circulatory physiology 2005 Apr;288(4):H1591-7
American journal of physiology. Heart and circulatory physiology 2005 Apr;288(4):H1591-7
Distinct signaling events downstream of mTOR cooperate to mediate the effects of amino acids and insulin on initiation factor 4E-binding proteins.
Wang X, Beugnet A, Murakami M, Yamanaka S, Proud CG
Molecular and cellular biology 2005 Apr;25(7):2558-72
Molecular and cellular biology 2005 Apr;25(7):2558-72
Translational control of cell fate: availability of phosphorylation sites on translational repressor 4E-BP1 governs its proapoptotic potency.
Li S, Sonenberg N, Gingras AC, Peterson M, Avdulov S, Polunovsky VA, Bitterman PB
Molecular and cellular biology 2002 Apr;22(8):2853-61
Molecular and cellular biology 2002 Apr;22(8):2853-61
Regulation of 4E-BP1 phosphorylation: a novel two-step mechanism.
Gingras AC, Gygi SP, Raught B, Polakiewicz RD, Abraham RT, Hoekstra MF, Aebersold R, Sonenberg N
Genes & development 1999 Jun 1;13(11):1422-37
Genes & development 1999 Jun 1;13(11):1422-37
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Supportive validation
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- antibodies-online (provider)
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- Experimental details
- Lysates prepared from NIH3T3 cells left untreated or treated with PI -3K inhibitor LY2904002 (lanes 2 & 3), prior to PDGF stimulation (lanes 1 & 3) and from EGF-treated HEK293 cells (4-8), Membranes were then left untreated (lanes 1-7) or treated with Lambda phosphatase (8), and incubated with 4E-BP1 [pT46] antibody for two hours at room temperature, following prior incubation with: no peptide (lanes 1 & 2), non- phosphopeptide corresponding to the immunogen (3), a generic phospho-threonine-containing peptide (4), or the phosphopeptide immunogen (5).
