Explore
Validate
Learn
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [11]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Other assay [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MA1-12607 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Fyn Monoclonal Antibody (1S)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- This antibody recognizes epitope aa 26-75. Staining of formalin-fixed paraffin embedded tissue sections requires boiling the sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at room temperature for 20 minutes.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 1S
- Vial size
- 100 µL
- Concentration
- 0.2 mg/mL
- Storage
- 4° C
Submitted references Modulating p56Lck in T-Cells by a Chimeric Peptide Comprising Two Functionally Different Motifs of Tip from Herpesvirus saimiri.
Interaction of myelin basic protein with cytoskeletal and signaling proteins in cultured primary oligodendrocytes and N19 oligodendroglial cells.
Epidermal hyperplasia and papillomatosis in mice with a keratinocyte-restricted deletion of csk.
In vitro modeling of human pancreatic duct epithelial cell transformation defines gene expression changes induced by K-ras oncogenic activation in pancreatic carcinogenesis.
Developmental partitioning of myelin basic protein into membrane microdomains.
Possible mechanisms underlying the protective effects of SY-21, an extract of a traditional Chinese herb, on transient brain ischemia/reperfusion-induced neuronal death in rat hippocampus.
Tyrosine kinase and tyrosine phosphatase participate in regulation of interactions of NMDA receptor subunit 2A with Src and Fyn mediated by PSD-95 after transient brain ischemia.
Tyrosine kinase and tyrosine phosphatase participate in regulation of interactions of NMDA receptor subunit 2A with Src and Fyn mediated by PSD-95 after transient brain ischemia.
Suppression of postsynaptic density protein 95 expression attenuates increased tyrosine phosphorylation of NR2A subunits of N-methyl-D-aspartate receptors and interactions of Src and Fyn with NR2A after transient brain ischemia in rat hippocampus.
Increased tyrosine phosphorylation of alpha(1C) subunits of L-type voltage-gated calcium channels and interactions among Src/Fyn, PSD-95 and alpha(1C) in rat hippocampus after transient brain ischemia.
Activation of NMDA receptors and L-type voltage-gated calcium channels mediates enhanced formation of Fyn-PSD95-NR2A complex after transient brain ischemia.
Vernot JP, Perdomo-Arciniegas AM, Pérez-Quintero LA, Martínez DF
Journal of immunology research 2015;2015:395371
Journal of immunology research 2015;2015:395371
Interaction of myelin basic protein with cytoskeletal and signaling proteins in cultured primary oligodendrocytes and N19 oligodendroglial cells.
Boggs JM, Homchaudhuri L, Ranagaraj G, Liu Y, Smith GS, Harauz G
BMC research notes 2014 Jun 24;7:387
BMC research notes 2014 Jun 24;7:387
Epidermal hyperplasia and papillomatosis in mice with a keratinocyte-restricted deletion of csk.
Honda K, Sakaguchi T, Sakai K, Schmedt C, Ramirez A, Jorcano JL, Tarakhovsky A, Kamisoyama H, Sakai T
Carcinogenesis 2007 Oct;28(10):2074-81
Carcinogenesis 2007 Oct;28(10):2074-81
In vitro modeling of human pancreatic duct epithelial cell transformation defines gene expression changes induced by K-ras oncogenic activation in pancreatic carcinogenesis.
Qian J, Niu J, Li M, Chiao PJ, Tsao MS
Cancer research 2005 Jun 15;65(12):5045-53
Cancer research 2005 Jun 15;65(12):5045-53
Developmental partitioning of myelin basic protein into membrane microdomains.
DeBruin LS, Haines JD, Wellhauser LA, Radeva G, Schonmann V, Bienzle D, Harauz G
Journal of neuroscience research 2005 Apr 15;80(2):211-25
Journal of neuroscience research 2005 Apr 15;80(2):211-25
Possible mechanisms underlying the protective effects of SY-21, an extract of a traditional Chinese herb, on transient brain ischemia/reperfusion-induced neuronal death in rat hippocampus.
Chen M, Wang Y, Liu Y, Hou XY, Zhang QG, Meng FJ, Zhang GY
Brain research 2003 Nov 7;989(2):180-6
Brain research 2003 Nov 7;989(2):180-6
Tyrosine kinase and tyrosine phosphatase participate in regulation of interactions of NMDA receptor subunit 2A with Src and Fyn mediated by PSD-95 after transient brain ischemia.
Chen M, Hou X, Zhang G
Neuroscience letters 2003 Mar 13;339(1):29-32
Neuroscience letters 2003 Mar 13;339(1):29-32
Tyrosine kinase and tyrosine phosphatase participate in regulation of interactions of NMDA receptor subunit 2A with Src and Fyn mediated by PSD-95 after transient brain ischemia.
Chen M, Hou X, Zhang G
Neuroscience letters 2003 Mar 13;339(1):29-32
Neuroscience letters 2003 Mar 13;339(1):29-32
Suppression of postsynaptic density protein 95 expression attenuates increased tyrosine phosphorylation of NR2A subunits of N-methyl-D-aspartate receptors and interactions of Src and Fyn with NR2A after transient brain ischemia in rat hippocampus.
Hou XY, Zhang GY, Zong YY
Neuroscience letters 2003 Jun 5;343(2):125-8
Neuroscience letters 2003 Jun 5;343(2):125-8
Increased tyrosine phosphorylation of alpha(1C) subunits of L-type voltage-gated calcium channels and interactions among Src/Fyn, PSD-95 and alpha(1C) in rat hippocampus after transient brain ischemia.
Hou XY, Zhang GY, Yan JZ, Liu Y
Brain research 2003 Jul 25;979(1-2):43-50
Brain research 2003 Jul 25;979(1-2):43-50
Activation of NMDA receptors and L-type voltage-gated calcium channels mediates enhanced formation of Fyn-PSD95-NR2A complex after transient brain ischemia.
Hou XY, Zhang GY, Yan JZ, Chen M, Liu Y
Brain research 2002 Nov 15;955(1-2):123-32
Brain research 2002 Nov 15;955(1-2):123-32
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on membrane enriched extracts (30 µg lysate) of HEK 293 (Lane 1), MCF7 (Lane 2) and Mouse Liver (Lane 3). The blot was probed with Anti-Fyn Mouse Monoclonal Antibody (Product # MA1-12607, 1-3 µg/mL) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.4 µg/mL, 1:2500 dilution). A 60 kDa band corresponding to Fyn was observed across the cell lines and tissue tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 10 % Bis-Tris gel (Product # NP0302BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody using iBind™ Flex Western Starter Kit (Product # SLF2000S). Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on membrane enriched extracts (30 µg lysate) of HEK 293 (Lane 1), MCF7 (Lane 2) and Mouse Liver (Lane 3). The blot was probed with Anti-Fyn Mouse Monoclonal Antibody (Product # MA1-12607, 1-3 µg/mL) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.4 µg/mL, 1:2500 dilution). A 60 kDa band corresponding to Fyn was observed across the cell lines and tissue tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 10 % Bis-Tris gel (Product # NP0302BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody using iBind™ Flex Western Starter Kit (Product # SLF2000S). Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Fyn in formalin-fixed, paraffin-embedded human lymphoma using a Fyn monoclonal antibody (Product # AHO0482). Detection was performed with a peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of tumor cells.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 7 Fyn detection in stimulated human PBMC. (a) PBMC were treated with PHA-P and hTip-CSKH during 2 h, lysed, and the protein extract resolved by SDS-PAGE. Western blot was performed with an anti-Fyn specific antibody. PHA-P was used as positive control, which induces the appearance of a second band of 56 kDa. (b) Quantification of the Fyn p59 and p56 bands. The intensity of each Fyn band was normalized to the loading control beta -actin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation and Western blot of Fyn was performed using mouse spleen cell lysates. Cells were lysed using ice cold IP lysis/wash buffer and pre-cleared using 50 mL of bead slurry per mL of cell lysate. Antigen-antibody complexes were formed by incubating 0.5 mL pre-cleared cell lysate on ice for 3hrs with 8-15 µg of Fyn monoclonal antibody (Product # AHO0482) crosslinked to Protein A/G plus agarose. The immune complexes were eluted using 60 µL sample buffer boiled at 95°C for 5 min and loaded onto an SDS-PAGE gel; Input (lane 1) and Jurkat IP (Lane 2). The membrane was probed with a Fyn monoclonal antibody (Product # AHO0482) at a dilution of 3 µg/mg of lysate followed by detection using an HRP-conjugated goat anti-mouse IgG + IgM (H+L) cross-adsorbed secondary antibody. Chemiluminescent detection was performed using an exposure time of 10m, resulting in a ~60 kDa band on input and IP lysates.
