H00002534-D01P

antibody from Abnova Corporation

Targeting: FYN MGC45350, SLK, SYN

Western blot (Supportive data in Antibodypedia)

Proximity ligation assay (Supportive data in Antibodypedia)

Antibody Data

Product number

H00002534-D01P

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00002534-D01P, RRID:AB_1673826

Product name

FYN purified MaxPab rabbit polyclonal antibody (D01P)

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against a full-length human FYN protein.

Antigen sequence

MGCVQCKDKEATKLTEERDGSLNQSSGYRYGTDPT
PQHYPSFGVTSIPNYNNFHAAGGQGLTVFGGVNSS
SHTGTLRTRGGTGVTLFVALYDYEARTEDDLSFHK
GEKFQILNSSEGDWWEARSLTTGETGYIPSNYVAP
VDSIQAEEWYFGKLGRKDAERQLLSFGNPRGTFLI
RESETTKGAYSLSIRDWDDMKGDHVKHYKIRKLDN
GGYYITTRAQFETLQQLVQHYSERAAGLCCRLVVP
CHKGMPRLTDLSVKTKDVWEIPRESLQLIKRLGNG
QFGEVWMGTWNGNTKVAIKTLKPGTMSPESFLEEA
QIMKKLKHDKLVQLYAVVSEEPIYIVTEYMNKGSL
LDFLKDGEGRALKLPNLVDMAAQVAAGMAYIERMN
YIHRDLRSANILVGNGLICKIADFGLARLIEDNEY
TARQGAKFPIKWTAPEAALYGRFTIKSDVWSFGIL
LTELVTKGRVPYPGMNNREVLEQVERGYRMPCPQD
CPISLHELMIHCWKKDPEERPTFEYLQSFLEDYFT
ATEPQYQPGENL


Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Analysis of protein-protein interactions in cross-talk pathways reveals CRKL protein as a novel prognostic marker in hepatocellular carcinoma.

Liu CH, Chen TC, Chau GY, Jan YH, Chen CH, Hsu CN, Lin KT, Juang YL, Lu PJ, Cheng HC, Chen MH, Chang CF, Ting YS, Kao CY, Hsiao M, Huang CY
Molecular & cellular proteomics : MCP 2013 May;12(5):1335-49

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

FYN MaxPab rabbit polyclonal antibody. Western Blot analysis of FYN expression in HeLa.

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of FYN expression in transfected 293T cell line (H00002534-T01) by FYN MaxPab polyclonal antibody.Lane 1: FYN transfected lysate(60.80 KDa).Lane 2: Non-transfected lysate.

Proximity ligation assay

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Proximity Ligation Analysis of protein-protein interactions between FYN and CTNNB1. HeLa cells were stained with anti-FYN rabbit purified polyclonal 1:1200 and anti-CTNNB1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Validation comment

In situ Proximity Ligation Assay (Cell)