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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
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Validation data
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- Product number
- MA5-15866 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Fyn Monoclonal Antibody (2H8)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- MA5-15866 targets FYN in WB applications and shows reactivity with Human and mouse samples.
- Antibody clone number
- 2H8
- Concentration
- Conc. Not Determined
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of FYN using FYN monoclonal antibody (Product # MA5-15866) in NIH/3T3 (1) and HeLa (2) cell lysate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of Fyn was achieved by transfecting HEK-293 with Fyn specific siRNA (Silencer® select Product # s5435). Western blot analysis (Fig. a) was performed using whole cell extracts from Fyn knockdown cells (Lane 3), non-specific scrambled siRNA transfected cells (Lane 2) and untransfected cells (Lane 1). The blot was probed Fyn Monoclonal Antibody (2H8) (Product # MA5-15866, 1:1000 dilution) and Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to Fyn.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Fyn Monoclonal Antibody (2H8) (Product # MA5-15866) and a 60 kDa band corresponding to Fyn was observed across cell lines and tissue extracts. Whole cell extracts (30 µg lysate) of MOLT-4 (Lane 1), Raji (Lane 2), Ramos (Lane 3), MCF7 (Lane 4), HEK-293 (Lane 5) and tissue extracts (30ug lysate) of Mouse Brain (Lane 6) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0302BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001).The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
