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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Flow cytometry [2]
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- Product number
- PA5-14780 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Fyn Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with bovine, chicken and rat based on sequence homology.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 μL
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references PAI-1-Dependent Inactivation of SMAD4-Modulated Junction and Adhesion Complex in Obese Endometrial Cancer.
Lin LL, Kost ER, Lin CL, Valente P, Wang CM, Kolonin MG, Daquinag AC, Tan X, Lucio N, Hung CN, Wang CP, Kirma NB, Huang TH
Cell reports 2020 Oct 13;33(2):108253
Cell reports 2020 Oct 13;33(2):108253
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Fyn in 293 cell lysates. Samples were incubated with Fyn polyclonal antibody (Product # PA5-14780). Lysates: 2 µg/lane. 293 cell lysates either nontransfected (Lane 1) or transiently transfected (Lane 2) with the FYN gene.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Fyn in mouse liver tissue. Samples were incubated with Fyn polyclonal antibody (Product # PA5-14780) followed by HRP-anti-rabbit (used for signal visualization with chemiluminescence). FYN (arrow).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Fyn in formalin-fixed and paraffin-embedded human cancer tissue. Samples were incubated with Fyn polyclonal antibody (Product # PA5-14780) which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of HeLa cells using a FYN polyclonal antibody (Product # PA5-14780) (right) compared to a negative control cell (left) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of Fyn in Hela cells (right histogram). Samples were incubated with Fyn polyclonal antibody (Product # PA5-14780) followed by FITC-conjugated goat-anti-rabbit secondary antibody. Negative control cell (left histogram).
