PA5-28975

antibody from Invitrogen Antibodies

Targeting: FYN MGC45350, SLK, SYN

Antibody data

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Validation data

FYN protein structure - PA5-28975 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of FYN in Mouse and rat tissue extracts (50 µg). Samples were separated by 10% SDS-PAGE and the membrane was probed with FYN Polyclonal antibody (Product # PA5-28975) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using Fyn Polyclonal Antibody (Product # PA5-28975). Sample (30 µg of whole cell lysate). Lane A: Jurkat. 10% SDS PAGE. Fyn Polyclonal Antibody (Product # PA5-28975) diluted at 1:1,000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fyn Polyclonal Antibody detects FYN protein by western blot analysis. Mouse and rat tissue extracts (50 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Fyn Polyclonal Antibody (Product # PA5-28975) diluted at 1:1,000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of Fyn was achieved by transfecting HEK-293 with Fyn specific siRNA (Silencer® select Product # s5435). Western blot analysis (Fig. a) was performed using whole cell extracts from Fyn knockdown cells (Lane 3), non-specific scrambled siRNA transfected cells (Lane 2) and untransfected cells (Lane 1). The blot was probed with Fyn Polyclonal Antibody (Product # PA5-28975, 1:1500 dilution) and Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 0.25µg/ml, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to Fyn.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Fyn Polyclonal Antibody (Product # PA5-28975) and 60 kDa, 45 kDa bands corresponding to Fyn was observed across cell lines and tissue extracts along with an uncharacterized band at ~ 20 kDa in the cell lines tested and at ~ 25 kDa in tissue. Whole cell extracts (30 µg lysate) of HEK-293 (Lane 1), MOLT-4 (Lane 2), Raji (Lane 3), Ramos (Lane 4) and tissue extracts (30ug lysate) of Rat Brain (Lane 5) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0302BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001).The blot was probed with the primary antibody (1:1500 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).