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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- ELISA [1]
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- Product number
- H00007224-M10 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00007224-M10, RRID:AB_10902596
- Product name
- TRPC5 monoclonal antibody (M10), clone 1C8
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a partial recombinant TRPC5.
- Antigen sequence
GLNQLYFYYETRAIDEPNNCKGIRCEKQNNAFSTL
FETLQSLFWSVFGLLNLYVTNVKARHEFTEFVGAT- Isotype
- IgG
- Antibody clone number
- 1C8
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of TRPC5 expression in transfected 293T cell line by TRPC5 monoclonal antibody (M10), clone 1C8.Lane 1: TRPC5 transfected lysate (Predicted MW: 111.4 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged TRPC5 is 1 ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
