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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- MA5-27657 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TRPC5 Monoclonal Antibody (N67/15)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- N67/15
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of TRPC5 in rat brain membrane with 20 µg of sample. The sample was blocked with 2% BSA and 2% skim milk in TBST, incubated with TRPC5 monoclonal antibody (Product # MA5-27657) using a dilution of 1:1000 (16 hours at 4°C), followed by Goat Anti-Mouse HRP (1 hour at RT) at a dilution of 1:100 and ECL development (6 min at RT). Samples were arranged as follows: Lane 1: Molecular Weight Ladder (MW), Lane 2: Rat Brain membrane cell lysate.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of TRPC5 in mouse brain. Samples were incubated with TRPC5 monoclonal antibody (Product # MA5-27657) using a dilution of 1:1000 (1 hour at RT) and followed by Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) and Mayer Hematoxylin (purple/blue) at a dilution of 250-500 µl. Magnification: 10X.
