Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- AF1744 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal C-Reactive Protein/CRP Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects rat C-Reactive Protein/CRP in direct ELISAs and Western blots. In Western blots, approximately 20% cross-reactivity with recombinant mouse CRP is observed and 10% cross-reactivity with recombinant human CRP is observed.
- Reactivity
- Rat
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Angiotensin II-induced non-alcoholic fatty liver disease is mediated by oxidative stress in transgenic TG(mRen2)27(Ren2) rats.
Novel differential neuroproteomics analysis of traumatic brain injury in rats.
Wei Y, Clark SE, Morris EM, Thyfault JP, Uptergrove GM, Whaley-Connell AT, Ferrario CM, Sowers JR, Ibdah JA
Journal of hepatology 2008 Sep;49(3):417-28
Journal of hepatology 2008 Sep;49(3):417-28
Novel differential neuroproteomics analysis of traumatic brain injury in rats.
Kobeissy FH, Ottens AK, Zhang Z, Liu MC, Denslow ND, Dave JR, Tortella FC, Hayes RL, Wang KK
Molecular & cellular proteomics : MCP 2006 Oct;5(10):1887-98
Molecular & cellular proteomics : MCP 2006 Oct;5(10):1887-98
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Rat C-Reactive Protein/CRP by Simple WesternTM. Simple Western lane view shows lysates of rat liver tissue, loaded at 0.2 mg/mL. A specific band was detected for C-Reactive Protein/CRP at approximately 34 kDa (as indicated) using 10 µg/mL of Goat Anti-Rat C-Reactive Protein/CRP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1744) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Rat C-Reactive Protein/CRP by Western Blot. Western blot shows lysates of rat liver tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Rat C-Reactive Protein/CRP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1744) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for C-Reactive Protein/CRP at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- C-Reactive Protein/CRP in Rat Liver. C-Reactive Protein/CRP was detected in perfusion fixed frozen sections of rat liver using Goat Anti-Rat C-Reactive Protein/CRP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1744) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of hepatocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.