Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- AF1707 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Sheep Polyclonal C-Reactive Protein/CRP Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human C-Reactive Protein/CRP in direct ELISAs and Western blots. In Western blots, approximately 35% cross-reactivity with recombinant mouse CRP and 10% cross-reactivity with recombinant rat CRP is observed.
- Reactivity
- Human
- Host
- Sheep
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human C-Reactive Protein/CRP by Western Blot. Western blot shows lysates of human kidney tissue . PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human C-Reactive Protein/CRP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1707) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for C-Reactive Protein/CRP at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- C-Reactive Protein/CRP in Human Kidney Cancer Tissue. C-Reactive Protein/CRP was detected in immersion fixed paraffin-embedded sections of human kidney cancer tissue using 1.7 µg/mL Human C-Reactive Protein/CRP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1707) overnight at 4 °C. Tissue was stained with the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific labeling was localized to epithelial cells in tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.