701245

antibody from Invitrogen Antibodies

Targeting: CRP PTX1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

701245

Provider

Invitrogen Antibodies

Product name

CRP Recombinant Rabbit Monoclonal Antibody (1H3L5)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Reactivity

Human

Host

Rabbit

Isotype

IgG

Antibody clone number

1H3L5

Vial size

100 µg

Concentration

0.5 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of recombinant CRP protein using a CRP recombinant rabbit monoclonal antibody (Product # 701245) at a dilution of 1 µg/mL. Samples were detected using chemiluminescence (ECL). Results show a band at ~23kDa.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of CRP was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with CRP Recombinant Rabbit Monoclonal Antibody (1H3L5) (Product # 701245, 5 µg/mL) in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790, 1:2000 dilution), for 45 minutes at room temperature (Panels a,e,i: Green). Nuclei (Panels b,f,j: Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panels c,g,k: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300 dilution). Panels d,h,l represents the merged images showing cytoplasmic localization upon treatment with IL-6 and PTI. Panel (a-d) shows representative Hep G2 control cells, whereas Panel (e-h) represent Hep G2 with Protein Transport Inhibitor Cocktail (PTI)(500X) (1X, 4 hours) treatment. Similarly, panel (i-l) represent Hep G2 cells treated with IL-6 and Protein Transport Inhibitor Cocktail (PTI)(500X) (10 ng/mL, 1X; 24 hours, 4 hours). The images were captured at 60X magnification.