Explore
Validate
Learn
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AM09005PU-S - Provider product page
- Provider
- OriGene
- Product name
- C Reactive Protein (CRP) (19-224) mouse monoclonal antibody, clone 5A9, Purified
- Antibody type
- Monoclonal
- Description
- C Reactive Protein (CRP) (19-224) mouse monoclonal antibody, clone 5A9, Purified
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- CRP
- Isotype
- IgG
- Antibody clone number
- 5A9
- Vial size
- 50 µl
- Concentration
- 1.0 mg/ml
No comments: Submit comment
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Cell lysates of NIH-3T3, HepG2, Jurkat, HeLa and SH-SY5Y (each 40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CRP (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis: Cell lysates of NIH-3T3 (lane 1), HepG2 (lane 2), Jurkat (lane 3), HeLa (lane 4) and SH-SY5Y (lane 5) -each 40 ug- were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CRP (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
- Validation comment
- WB
