710269

antibody from Invitrogen Antibodies

Targeting: CRP PTX1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

710269

Provider

Invitrogen Antibodies

Product name

CRP Recombinant Polyclonal Antibody (5HCLC)

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

This antibody is predicted to react with non-human primate, rat and rabbit based on sequence homology.

Antibody clone number

5HCLC

Concentration

0.5 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-CRP Recombinant Polyclonal Antibody (5HCLC) (Product # 710269) and a 25 kDa band corresponding to C-reactive protein was observed in HepG2 upon IL-6 and PTI treatment. Whole cell extracts (60 µg lysate) of Hep G2 (Lane 1), Hep G2 (Protein Transport Inhibitor Cocktail (500X) (1X, 4 hours)) (Lane 2), Hep G2 (IL-6 and Protein Transport Inhibitor Cocktail (500X) (10 ng/mL, 1X; 24 hours,4 hours)) (Lane 3) were electrophoresed using NuPAGE™ 12% Bis-Tris Protein Gel (Product # NP0341BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1 µg/mL) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:4000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Atto Ultimate Sensitivity Substrate (Product # A38556).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of CRP in whole cell extracts from NIH-3T3 cells using a CRP Recombinant Rabbit Polyclonal Antibody (Product # 710269) at a dilution of 1 µg/mL. Samples were detected using chemiluminescence (ECL). Results show a band at ~25kDa.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of C-Reactive Protein showing staining in the cytoplasm and weak staining in the nucleus of paraffin-embedded human liver tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a C-Reactive Protein Recombinant Rabbit Polyclonal Antibody (Product # 710269) diluted in 3% BSA-PBS at a dilution of 1:20 for 1 hour at 37ºC in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.