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ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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- Product number
- AM09005PU-S - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AM09005PU-S, RRID:AB_1614439
- Product name
- anti C-reactive protein (CRP)
- Antibody type
- Monoclonal
- Antigen
- Recombinant human CRP (19-224 aa) purified from E. coli
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 5A9
- Vial size
- 50 µl
- Concentration
- 1.0 mg/ml
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis: Cell lysates of NIH-3T3 (lane 1), HepG2 (lane 2), Jurkat (lane 3), HeLa (lane 4) and SH-SY5Y (lane 5) -each 40 ug- were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CRP (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Cell lysates of NIH-3T3, HepG2, Jurkat, HeLa and SH-SY5Y (each 40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CRP (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
