Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- ABIN1881718 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1) (N-Term), (AA 16-47) antibody
- Antibody type
- Polyclonal
- Antigen
- This Rat Prkaa1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 16-47 amino acids from the N-terminal region of rat Prkaa1.
- Description
- This antibody is purified through a protein A column, followed by peptide affinity purification. Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Epitope
- N-Term,AA 16-47
- Vial size
- 400 μL
- Concentration
- 0.44 mg/mL
Submitted references Characterization of the AMP-activated protein kinase kinase from rat liver and identification of threonine 172 as the major site at which it phosphorylates AMP-activated protein kinase.
Mammalian 5'-AMP-activated protein kinase non-catalytic subunits are homologs of proteins that interact with yeast Snf1 protein kinase.
Regulation of HMG-CoA reductase: identification of the site phosphorylated by the AMP-activated protein kinase in vitro and in intact rat liver.
Hawley SA, Davison M, Woods A, Davies SP, Beri RK, Carling D, Hardie DG
The Journal of biological chemistry 1996 Nov 1;271(44):27879-87
The Journal of biological chemistry 1996 Nov 1;271(44):27879-87
Mammalian 5'-AMP-activated protein kinase non-catalytic subunits are homologs of proteins that interact with yeast Snf1 protein kinase.
Stapleton D, Gao G, Michell BJ, Widmer J, Mitchelhill K, Teh T, House CM, Witters LA, Kemp BE
The Journal of biological chemistry 1994 Nov 25;269(47):29343-6
The Journal of biological chemistry 1994 Nov 25;269(47):29343-6
Regulation of HMG-CoA reductase: identification of the site phosphorylated by the AMP-activated protein kinase in vitro and in intact rat liver.
Clarke PR, Hardie DG
The EMBO journal 1990 Aug;9(8):2439-46
The EMBO journal 1990 Aug;9(8):2439-46
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Supportive validation
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- antibodies-online (provider)
- Main image
- Experimental details
- Rat Prkaa1 Antibody (N-term) (ABIN1881718) western blot analysis in CEM,mouse NIH/3T3 cell line and rat cerebellum tissue lysates (35 μg/lane). This demonstrates the rat Prkaa1 antibody detected the rat Prkaa1 protein (arrow).