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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [3]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-36045 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- AMPK alpha-1,2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody detects endogenous protein at a molecular weight of 63 kDa.
- Concentration
- 1 mg/mL
Submitted references Schistosome AMPK Is Required for Larval Viability and Regulates Glycogen Metabolism in Adult Parasites.
Metformin Impairs Glutamine Metabolism and Autophagy in Tumour Cells.
Host Adaptive Immune Status Regulates Expression of the Schistosome AMP-Activated Protein Kinase.
Hunter KS, Miller A, Mentink-Kane M, Davies SJ
Frontiers in microbiology 2021;12:726465
Frontiers in microbiology 2021;12:726465
Metformin Impairs Glutamine Metabolism and Autophagy in Tumour Cells.
Saladini S, Aventaggiato M, Barreca F, Morgante E, Sansone L, Russo MA, Tafani M
Cells 2019 Jan 14;8(1)
Cells 2019 Jan 14;8(1)
Host Adaptive Immune Status Regulates Expression of the Schistosome AMP-Activated Protein Kinase.
Hunter KS, Davies SJ
Frontiers in immunology 2018;9:2699
Frontiers in immunology 2018;9:2699
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of AMPK alpha-1,2 in Lane 1: HeLa cell lysate, Lane 2: mouse brain tissue lysate, Lane 3: rat brain tissue lysate. Samples were incubated with AMPK alpha-1,2 polyclonal antibody (Product # PA5-36045) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of AMPK alpha-1,2 in Lane 1: HeLa cell lysate, Lane 2: mouse brain tissue lysate, Lane 3: rat brain tissue lysate. Samples were incubated with AMPK alpha-1,2 polyclonal antibody (Product # PA5-36045) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of AMPK alpha-1+2 using AMPK alpha-1+2 polyclonal antibody (Product # PA5-36045) at a dilution of 1:500. Lane 1: Hela cell lysate, Lane 2: Mouse brain tissue lysate, Lane 3: Rat brain tissue lysate.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of AMPK alpha-1,2 in paraffin-embedded human breast carcinoma tissue. Samples were incubated with AMPK alpha-1,2 polyclonal antibody (Product # PA5-36045) at a dilution of 1:100.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- FIGURE 2 Dorsomorphin inhibits S. mansoni AMPK alpha kinase activity. (A) AMPK alpha was immunoprecipitated from lysates of freshly isolated adult schistosomes using anti-AMPK alpha, then resuspended in 1x kinase buffer with 0.2 mug acetyl-coA-carboxylase (ACC) in either the presence or absence of 10 muM Dorsomorphin. Control kinase assays were performed where the lysate (source of the kinase) was omitted. Kinase assay supernatants were probed by Western blot using anti-phospho ACC and anti-ACC antibodies. IP indicates antibody used for immunoprecipitation. WB indicates the antibody used for Western blotting. (B) The intensity of the anti-phospho ACC signal relative to the anti-ACC signal for each sample was determined. Each data point represents an independent kinase reaction. ** P
