Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-36615 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-AMPK alpha-1 (Ser486) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody detects endogenous protein at a molecular weight of 63 kDa. It does not cross-react with phosphorylated AMPK alpha-2 or other related proteins. Purity is >95% by SDS-PAGE.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-AMPK alpha-1 pSer486 using Phospho-AMPK alpha-1 pSer486 polyclonal antibody (Product # PA5-36615) at a dilution of 1:500. Lane 1: HEK293T cell lysate treated with UV, Lane 2: sp2/0 cell lysate treated with UV, Lane 3: PC12 cell lysate treated with UV.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-AMPK alpha-1 (Ser486) in Lane 1: L02 whole cell lysate, Lane 2: L02 treated with PBS (1×PBS,PH7.4) for 30min whole cell lysate, Lane 3: L02 treated with PBS (1×PBS,PH7.4) for 60min whole cell lysate, Lane 4: L02 treated with PBS (1×PBS,PH7.4) for 120min whole cell lysate, Lane 5: L02 treated with PBS (1×PBS,PH7.4) for 180min whole cell lysate, Lane 6: L02 treated with PBS (1×PBS,PH7.4) for 240min whole cell lysate, Lane 7: PC12 whole cell lysate. Samples were incubated with Phospho-AMPK alpha-1 (Ser486) polyclonal antibody (Product # PA5-36615) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-AMPK alpha-1 (Ser486) in Lane 1: HEK293T cell lysate treated with UV, Lane 2: sp2/0 cell lysate treated with UV, Lane 3: PC12 cell lysate treated with UV. Samples were incubated with Phospho-AMPK alpha-1 (Ser486) polyclonal antibody (Product # PA5-36615) at a dilution of 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Phospho-AMPK alpha-1 pSer486 in paraffin-embedded human colorectal carcinoma using Phospho-AMPK alpha-1 pSer486 polyclonal antibody (Product # PA5-36615) at a dilution of 1:50.