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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- ELISA [3]
- Immunohistochemistry [2]
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Validation data
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- Product number
- LS-C359330 - Provider product page
- Provider
- LSBio
- Product name
- PRKAA1 / AMPK Alpha 1 Antibody (clone 2B7) LS-C359330
- Antibody type
- Monoclonal
- Description
- Ammonium sulfate precipitation
- Reactivity
- Human, Mouse, Rat, Simian
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 2B7
- Storage
- Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
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Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western Blot: AMPK alpha 1 Antibody (2B7) - Western blot analysis using AMPK alpha 1 mouse mAb against Jurkat (1), HeLa (2), HepG2 (3), MCF-7 (4), Cos7 (5), NIH/3T3 (6), K562 (7), HEK293 (8), and PC-12 (9) cell lysate.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western Blot: AMPK alpha 1 Antibody (2B7) - Western blot shows a specific band for AMPK Alpha 1 in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- ELISA: AMPK alpha 1 Antibody (2B7) - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng).
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- ELISA: AMPK alpha 1 Antibody (2B7) - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng).
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- ELISA: AMPK alpha 1 Antibody (2B7) - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng).
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: AMPK alpha 1 Antibody (2B7) - Immunohistochemical analysis of paraffin-embedded ovarian cancer (left) and brain tissues (right) using AMPK alpha 1 mouse mAb with DAB staining.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: AMPK alpha 1 Antibody (2B7) - Immunohistochemical analysis of paraffin-embedded ovarian cancer (left) and brain tissues (right) using AMPK alpha 1 mouse mAb with DAB staining.
