Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
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Validation data
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- Product number
- LS-B14414 - Provider product page
- Provider
- LSBio
- Product name
- IHC-plus™ PRKAA1 / AMPK Alpha 1 Antibody (aa350-400) LS-B14414
- Antibody type
- Polyclonal
- Description
- Affinity purified
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Storage
- Store at 2-8°C for up to one year.
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Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Detection of Human, Rat, and Bovine AMPK alpha 1 by Western Blot. Samples: Extracts from 1. bovine aortic endothelial cells, 2. rat aortic smooth muscle cells, 3. HepG2 cells, or 4. human aortic endothelial cells. Antibody: Affinity purified rabbit anti-AMPK alpha 1 used at 2 ug/ml. Detection: Chemiluminescence.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Detection of human and mouse AMPK alpha 1 by western blot. Samples: Whole cell lysate (15 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-AMPK alpha 1 antibody used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.