Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- A00994-3 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-AMPK alpha 1/PRKAA1 Antibody Picoband™
- Antibody type
- Polyclonal
- Description
- Rabbit IgG polyclonal antibody for AMPK alpha 1 detection. Tested with WB, ICC/IF, FCM, Direct ELISA in Human;Mouse;Rat.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- 0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Submitted references Fructus Zanthoxyli extract improves glycolipid metabolism disorder of type 2 diabetes mellitus via activation of AMPK/PI3K/Akt pathway: Network pharmacology and experimental validation.
The effects of Formoterol in preventing adipogenesis and obesity are mediated by PPARγ/C/EBPα axis and AMPK/PGC-1α pathway.
The Function behind the Relation between Lipid Metabolism and Vimentin on H9N2 Subtype AIV Replication.
ROS -mediated p53 activation by juglone enhances apoptosis and autophagy in vivo and in vitro.
Zhang T, Zhang Q, Zheng W, Tao T, Li RL, Wang LY, Peng W, Wu CJ
Journal of integrative medicine 2022 Nov;20(6):543-560
Journal of integrative medicine 2022 Nov;20(6):543-560
The effects of Formoterol in preventing adipogenesis and obesity are mediated by PPARγ/C/EBPα axis and AMPK/PGC-1α pathway.
Zhang X, Che L, Shan J, Wang Y, Jia Y, Wu H
Bioscience, biotechnology, and biochemistry 2022 Jul 4;
Bioscience, biotechnology, and biochemistry 2022 Jul 4;
The Function behind the Relation between Lipid Metabolism and Vimentin on H9N2 Subtype AIV Replication.
Lu A, Yang J, Huang X, Huang X, Yin G, Cai Y, Feng X, Zhang X, Li Y, Liu Q
Viruses 2022 Aug 18;14(8)
Viruses 2022 Aug 18;14(8)
ROS -mediated p53 activation by juglone enhances apoptosis and autophagy in vivo and in vitro.
Wang P, Zhang SD, Jiao J, Wang W, Yu L, Zhao XL, Wang LT, Meng D, Fu YJ
Toxicology and applied pharmacology 2019 Sep 15;379:114647
Toxicology and applied pharmacology 2019 Sep 15;379:114647
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Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- Western blot analysis of AMPK alpha 1 using anti-AMPK alpha 1 antibody (A00994-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human PANC-1 whole cell lysates, Lane 2: human Jurkat whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AMPK alpha 1 antigen affinity purified polyclonal antibody (Catalog # A00994-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AMPK alpha 1 at approximately 63KD. The expected band size for AMPK alpha 1 is at 63KD.
- Additional image