STJ114591

antibody from St John's Laboratory

Targeting: PRKAA1 AMPKa1

Immunocytochemistry

Antibody data

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Validation data

Product number: STJ114591 - Provider product page
Provider: St John's Laboratory
Product name: Anti-PRKAA1/PRKAA2 antibody (STJ114591)
Antibody type: Polyclonal
Description: Rabbit polyclonal antibody anti-AMPKa1/AMPKa2 is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Antigen sequence: NA
Epitope: NA
Isotype: IgG
Antibody clone number: NA
Vial size: NA
Concentration: NA
Storage: Store in a freezer at-20°C and avoid freeze-thaw cycles.
Handling: NA

PRKAA1 protein structure - STJ114591 shown in red.

Supportive validation

Submitted by: St John's Laboratory (provider)
Main image
Experimental details: Immunofluorescence analysis of NIH/3T3 cells using AMPKa1/AMPKa2 rabbit polyclonal antibody (STJ114591) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
Sample type: NA
Validation comment: NA
Primary Ab dilution: NA
Other comments: NA
Secondary Ab: NA
Secondary Ab dilution: NA
Protocol: NA
Appendix: NA

Supportive validation

Submitted by: St John's Laboratory (provider)
Main image
Experimental details: Immunofluorescence analysis of PC-12 cells using AMPKa1/AMPKa2 rabbit polyclonal antibody (STJ114591) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
Sample type: NA
Validation comment: NA
Primary Ab dilution: NA
Other comments: NA
Secondary Ab: NA
Secondary Ab dilution: NA
Protocol: NA
Appendix: NA

Supportive validation

Submitted by: St John's Laboratory (provider)
Main image
Experimental details: Immunofluorescence analysis of U2OS cells using AMPKa1/AMPKa2 rabbit polyclonal antibody (STJ114591) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
Sample type: NA
Validation comment: NA
Primary Ab dilution: NA
Other comments: NA
Secondary Ab: NA
Secondary Ab dilution: NA
Protocol: NA
Appendix: NA

Supportive validation

Submitted by: St John's Laboratory (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded human colon using AMPKa1/AMPKa2 rabbit polyclonal antibody (STJ114591) at dilution of 1:25 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
Sample type: NA
Validation comment: NA
Primary Ab dilution: NA
Other comments: NA
Secondary Ab: NA
Secondary Ab dilution: NA
Protocol: NA
Appendix: NA

Supportive validation

Submitted by: St John's Laboratory (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded mouse kidney using AMPKa1/AMPKa2 rabbit polyclonal antibody (STJ114591) at dilution of 1:25 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
Sample type: NA
Validation comment: NA
Primary Ab dilution: NA
Other comments: NA
Secondary Ab: NA
Secondary Ab dilution: NA
Protocol: NA
Appendix: NA

Supportive validation

Submitted by: St John's Laboratory (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded rat lung using AMPKa1/AMPKa2 rabbit polyclonal antibody (STJ114591) at dilution of 1:25 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
Sample type: NA
Validation comment: NA
Primary Ab dilution: NA
Other comments: NA
Secondary Ab: NA
Secondary Ab dilution: NA
Protocol: NA
Appendix: NA