ABIN362185

antibody from antibodies-online

Targeting: PRKAA1 AMPKa1

Western blot (Recommended by provider)

Immunocytochemistry (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

ABIN362185

Provider

antibodies-online

Product name

anti-Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1) (pSer487) antibody

Antibody type

Polyclonal

Antigen

Peptide sequence around phosphorylation site of pSer487 (S-G- SP-V-S) derived from Human AMPKa1. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Description

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Epitope

pSer487

Isotype

IgG

Vial size

50 μL

Concentration

1 mg/mL

Storage

Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

References

Submitted references

Construction of adiponectin-encoding plasmid DNA and overexpression in mice in vivo.

Huang YN, Qi JH, Xiang L, Wang YZ
Gene 2012 Jul 10;502(2):87-93

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Global phosphoproteome of HT-29 human colon adenocarcinoma cells.

Kim JE, Tannenbaum SR, White FM
Journal of proteome research 2005 Jul-Aug;4(4):1339-46

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Identification of phosphorylation sites in AMP-activated protein kinase (AMPK) for upstream AMPK kinases and study of their roles by site-directed mutagenesis.

Woods A, Vertommen D, Neumann D, Turk R, Bayliss J, Schlattner U, Wallimann T, Carling D, Rider MH
The Journal of biological chemistry 2003 Aug 1;278(31):28434-42