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- Product number
- PA5-105297 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- AMPK alpha Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Antibody detects endogenous levels of total AMPK alpha.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C
Submitted references β-Nicotinamide Mononucleotide (NMN) Administrated by Intraperitoneal Injection Mediates Protection Against UVB-Induced Skin Damage in Mice.
Zhou X, Du HH, Long X, Pan Y, Hu J, Yu J, Zhao X
Journal of inflammation research 2021;14:5165-5182
Journal of inflammation research 2021;14:5165-5182
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of AMPK alpha in 293 cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C) incubated with AMPK alpha polyclonal antibody (Product # PA5-105297) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 at a dilution of 1:600.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of AMPK alpha in 293 cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C) incubated with AMPK alpha polyclonal antibody (Product # PA5-105297) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 at a dilution of 1:600.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 6 Protein expression of AMPK, NFkappaB-p65, IkappaB-alpha, SOD1 and CAT in skin tissues. ( A ) relative expression levels of proteins; ( B ) protein banding map. * p < 0.05 compared to the UVB group; ** p < 0.01 compared to the UVB group; *** p < 0.001 compared to the UVB group. Abbreviations : VC+UVB, mice treated with vitamin C(300mg/kg) and UVB irradiation; NMN+UVB, mice treated with nicotinamide mononucleotide (300mg/kg) and UVB irradiation.
