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- Antibody Data
- Antigen structure
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- Western blot [1]
- Flow cytometry [1]
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- Product number
- RQ6111 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- PRKAA1 Antibody / AMPK alpha 1
- Antibody type
- Polyclonal
- Description
- This highly specific PRKAA1 antibody is suitable for use in Western blot/Flow cytometry/Direct ELISA applications with human, mouse and rat samples.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the PRKAA1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) human Jurkat, 2) human HeLa, 3) human MCF7, 4) monkey COS-7, 5) human K562, 6) HEK293, 7) rat brain, 8) rat heart, 9) mouse brain, 10) mouse heart and 11) mouse intestine lysate with PRKAA1 antibody. Predicted molecular weight: 64-66 kDa (two isoforms).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human SiHa cells with PRKAA1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PRKAA1 antibody.
