MA5-14922

antibody from Invitrogen Antibodies

Targeting: PRKAA1 AMPKa1

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-14922

Provider

Invitrogen Antibodies

Product name

AMPK alpha-1 Monoclonal Antibody (C.961.4)

Antibody type

Monoclonal

Antigen

Purifed from natural sources

Description

It is not recommended to aliquot this antibody.

Reactivity

Human, Mouse, Rat, Hamster

Host

Mouse

Isotype

IgG

Antibody clone number

C.961.4

Vial size

100 µL

Concentration

1602 µg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on whole cell extracts (30 µg lysate) of MCF7 (Lane 1), MDA-MB-231 (Lane 2), LNCaP (Lane 3), PC-3 (Lane 4), NIH/3T3 (Lane 5), HEK-293 (Lane 6), Hep G2 (Lane 7) and A-431 (Lane 8). The blot was probed with Anti-AMPK alpha-1 Monoclonal Antibody (Product # MA5-14922, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/mL, 1:4000 dilution). A 63 kDa band corresponding to AMPK alpha-1 was observed across the cell lines tested.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of AMPK-alpha in extracts from HepG2 and 293 cells using AMPK-alpha monoclonal antibody (Product # MA5-14922).