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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- Ab088655 - Provider product page
- Provider
- Aladdin Scientific
- Product name
- Recombinant AMPK alpha 1 Antibody
- Antibody type
- Recombinant
- Description
- Rabbit anti Human AMPK alpha 1 Antibody, Recombinant, could be used for WB, IHC, IF, ICC and so on.Applicationá WB: 1/500-1/1000á IHC: 1/50-1/100á IF/ICC 1/50-1/200Protein FunctionResponsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antigen sequence
AA 500-559- Vial size
- 100_l,10_l,1ml,50_l
- Concentration
- 1,2 mg/ml
- Storage
- Store at 4¡C short term (1-2 weeks). Store at -20¡C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
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Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Recombinant AMPK alpha 1 Antibody (Ab088655) - Western Blot All lanes: Recombinant AMPK alpha 1 Antibody (Ab088655) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 62 kDa Observed band size: 66 kDa Exposure time: 5.0 s
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Recombinant AMPK alpha 1 Antibody (Ab088655) - IF/ICC Immunocytochemistry analysis of AMPK alpha 1 (green) in HeLa cells using Recombinant AMPK alpha 1 Antibody (Ab088655), and cells were counterstained with DAPI (blue).
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Recombinant AMPK alpha 1 Antibody (Ab088655) - IHC Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using Recombinant AMPK alpha 1 Antibody (Ab088655). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
