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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Flow cytometry [1]
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- Product number
- MA5-53544 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-AMPK alpha-1 (Ser496) Recombinant Rabbit Monoclonal Antibody (23GB2660)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- 23GB2660
- Vial size
- 100 μL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of AMPK alpha-1. Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with AMPK alpha-1 recombinant monoclonal antibody (Product # MA5-53544) with a dilution of 1:5,000, followed by HRP-conjugated goat anti-rabbit secondary antibody with a dilution of 1:20,000. Image was developed using FeQTM ECL Substrate Kit.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of AMPK alpha-1 in HeLa cells. Nuclei were stained blue with DAPI; AMPK alpha-1 recombinant monoclonal antibody (Product # MA5-53544) with a dilution of 1:1,000 was stained magenta with Alexa Fluor 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of AMPK alpha-1 in HeLa cells using AMPK alpha-1 recombinant monoclonal antibody (Product # MA5-53544) at a dilution of 1:2,000. Green, isotype control; red, AMPK alpha-1.
