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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Other assay [1]
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- Product number
- PA5-17398 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- AMPK alpha-1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- It is not recommended to aliquot this antibody. This antibody is not cross-reactive with AMPK-alpha2.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 139 µg/mL
- Storage
- -20°C
Submitted references Expression and Impact of Vaspin on In Vitro Oocyte Maturation through MAP3/1 and PRKAA1 Signalling Pathways.
Kurowska P, Mlyczyńska E, Estienne A, Barbe A, Rajska I, Soból K, Poniedziałek-Kempny K, Dupont J, Rak A
International journal of molecular sciences 2020 Dec 8;21(24)
International journal of molecular sciences 2020 Dec 8;21(24)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of AMPK-alpha1 in extracts from HeLa and HT29 cells using AMPK-alpha1 polyclonal antibody (Product # PA5-17398).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 4 Effect of vaspin on mitogen-activated kinase (MAP3/1) and AMP-activated kinase (PRKAA1) phosphorylation in oocytes and cumulus cells after in vitro maturation. COCs (50/group/experiment) were selected after morphological examination of material collected from the ovaries taken from 100 pigs (15 follicles/ovary). Porcine cumulus-oocyte complex (COCs) were cultured for 44 h in maturation medium in the presence or absence of vaspin (1 ng/mL) then mechanically separated into oocytes and cumulus cells. Phospho MAP3/1 (pMAP3/1), MAP3/1 ( A ) and phospho PRKAA1 (pPRKAA1), PRKAA1 ( B ) protein expressions were analysed by Western blot analysis. Protein expression levels were normalised to actin. Experiments were performed independently and repeated three times ( n = 3). Data are plotted as the mean +- SEM of three independent experiments. Significance between control and vaspin is indicated by ** p < 0.01 and *** p < 0.001.
