Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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Validation data
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- Product number
- MA5-15024 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NCK1 Monoclonal Antibody (K.349.1)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- It is not recommended to aliquot this antibody.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- K.349.1
- Vial size
- 100 µL
- Concentration
- 282 µg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of NCK1 was achieved by transfecting HEK-293 cells with NCK1 specific siRNAs (Silencer® select Product # s9310, s9309). Western blot analysis (Fig. a) was performed using whole cell extracts from the NCK1 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with Anti-NCK1 Monoclonal Antibody (Product # MA5-15024, 1:1000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to NCK1.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extracts (30 µg lysate) of A431 (Lane 1), HeLa (Lane 2), HEK293 (Lane 3), Jurkat (Lane 4), K562 (Lane 5), COS7 (Lane 6), U-87 MG (Lane 7) and Mouse Testis (Lane 8). The blot was probed with Anti-NCK1 Monoclonal Antibody (K.349.1) (Product # MA5-15024, 1:5000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 45kDa band corresponding to NCK1 was seen in all cell lines and tissue lysate tested.