LS-C330807

antibody from LSBio

Targeting: NCK1 NCK, NCKalpha

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

LS-C330807

Provider

LSBio

Product name

NCK1 / NCK Antibody LS-C330807

Antibody type

Polyclonal

Description

Affinity purified

Reactivity

Human

Host

Rabbit

Isotype

IgG

Storage

Store at 4°C, avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Western blot analysis of extracts of various cell lines, using NCK1 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 30s.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Western blot analysis of extracts of various cell lines, using NCK1 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 30s.