H00004690-D01P

antibody from Abnova Corporation

Targeting: NCK1 NCK, NCKalpha

Western blot (Supportive data in Antibodypedia)

Proximity ligation assay (Supportive data in Antibodypedia)

Antibody Data

Product number

H00004690-D01P

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00004690-D01P, RRID:AB_1677036

Product name

NCK1 purified MaxPab rabbit polyclonal antibody (D01P)

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against a full-length human NCK1 protein.

Antigen sequence

MAEEVVVVAKFDYVAQQEQELDIKKNERLWLLDDS
KSWWRVRNSMNKTGFVPSNYVERKNSARKASIVKN
LKDTLGIGKVKRKPSVPDSASPADDSFVDPGERLY
DLNMPAYVKFNYMAEREDELSLIKGTKVIVMEKCS
DGWWRGSYNGQVGWFPSNYVTEEGDSPLGDHVGSL
SEKLAAVVNNLNTGQVLHVVQALYPFSSSNDEELN
FEKGDVMDVIEKPENDPEWWKCRKINGMVGLVPKN
YVTVMQNNPLTSGLEPSPPQCDYIRPSLTGKFAGN
PWYYGKVTRHQAEMALNERGHEGDFLIRDSESSPN
DFSVSLKAQGKNKHFKVQLKETVYCIGQRKFSTME
ELVEHYKKAPIFTSEQGEKLYLVKHLS


Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of NCK1 expression in transfected 293T cell line (H00004690-T01) by NCK1 MaxPab polyclonal antibody.Lane 1: NCK1 transfected lysate(42.90 KDa).Lane 2: Non-transfected lysate.

Proximity ligation assay

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Proximity Ligation Analysis of protein-protein interactions between NCK1 and ABL2. HeLa cells were stained with anti-NCK1 rabbit purified polyclonal 1:1200 and anti-ABL2 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Validation comment

In situ Proximity Ligation Assay (Cell)