H00004690-B01P

antibody from Abnova Corporation

Targeting: NCK1 NCK, NCKalpha

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00004690-B01P

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00004690-B01P, RRID:AB_11183357

Product name

NCK1 purified MaxPab mouse polyclonal antibody (B01P)

Antibody type

Polyclonal

Description

Mouse polyclonal antibody raised against a full-length human NCK1 protein.

Antigen sequence

MAEEVVVVAKFDYVAQQEQELDIKKNERLWLLDDS
KSWWRVRNSMNKTGFVPSNYVERKNSARKASIVKN
LKDTLGIGKVKRKPSVPDSASPADDSFVDPGERLY
DLNMPAYVKFNYMAEREDELSLIKGTKVIVMEKCS
DGWWRGSYNGQVGWFPSNYVTEEGDSPLGDHVGSL
SEKLAAVVNNLNTGQVLHVVQALYPFSSSNDEELN
FEKGDVMDVIEKPENDPEWWKCRKINGMVGLVPKN
YVTVMQNNPLTSGLEPSPPQCDYIRPSLTGKFAGN
PWYYGKVTRHQAEMALNERGHEGDFLIRDSESSPN
DFSVSLKAQGKNKHFKVQLKETVYCIGQRKFSTME
ELVEHYKKAPIFTSEQGEKLYLVKHLS


Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of NCK1 expression in transfected 293T cell line (H00004690-T01) by NCK1 MaxPab polyclonal antibody.Lane 1: NCK1 transfected lysate(41.47 KDa).Lane 2: Non-transfected lysate.

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

NCK1 MaxPab polyclonal antibody. Western Blot analysis of NCK1 expression in A-431.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of purified MaxPab antibody to NCK1 on HeLa cell. [antibody concentration 20 ug/ml]

Validation comment

Immunofluorescence

Protocol

Protocol