Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
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Validation data
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- Product number
- H00004690-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00004690-M01, RRID:AB_2150441
- Product name
- Mouse Monoclonal NCK1 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. NCK1 - NCK adaptor protein 1
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Once phosphorylated, tyrosines in carboxyl terminus of protein-tyrosine kinase Syk interact with signaling proteins, including TULA-2, a negative regulator of mast cell degranulation.
de Castro RO, Zhang J, Groves JR, Barbu EA, Siraganian RP
The Journal of biological chemistry 2012 Mar 9;287(11):8194-204
The Journal of biological chemistry 2012 Mar 9;287(11):8194-204
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: NCK1 Antibody (1A1) [H00004690-M01] - Analysis of NCK1 expression in transfected 293T cell line by NCK1 monoclonal antibody (M01), clone 1A1.Lane 1: NCK1 transfected lysate(42.9 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: NCK1 Antibody (1A1) [H00004690-M01] - NCK1 monoclonal antibody (M01), clone 1A1 Analysis of NCK1 expression in Hela S3 NE.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: NCK1 Antibody (1A1) [H00004690-M01] - Detection limit for recombinant GST tagged NCK1 is approximately 0.1ng/ml as a capture antibody.