MA5-34616

antibody from Invitrogen Antibodies

Targeting: NCK1 NCK, NCKalpha

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-34616

Provider

Invitrogen Antibodies

Product name

NCK1 Recombinant Rabbit Monoclonal Antibody (JU41-80)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

Positive Control: NIH-3T3, Hela, rat kidney tissue lysate, mouse testis tissue lysate, A431, HUVEC, LOVO, rat testis tissue, human tonsil tissue, human kidney tissue.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Antibody clone number

JU41-80

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles, store in dark

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of NCK1 in Lane 1: HeLa, Lane 2: NIH-3T3, Lane 3: Rat kidney tissue, Lane 4: Mouse testis tissue. Samples were incubated with NCK1 monoclonal antibody (Product # MA5-34616), at a dilution of 1:500.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of NCK1 in LOVO cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with NCK1 monoclonal antibody (Product # MA5-34616), followed by DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of NCK1 in HUVEC cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with NCK1 monoclonal antibody (Product # MA5-34616), followed by DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of NCK1 in A431 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with NCK1 monoclonal antibody (Product # MA5-34616), followed by DAPI (blue).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of NCK1 in paraffin-embedded human tonsil tissue. Samples were incubated with NCK1 monoclonal antibody (Product # MA5-34616), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of NCK1 in paraffin-embedded rat testis tissue. Samples were incubated with NCK1 monoclonal antibody (Product # MA5-34616), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of NCK1 in paraffin-embedded mouse testis tissue. Samples were incubated with NCK1 monoclonal antibody (Product # MA5-34616), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of NCK1 in paraffin-embedded human kidney tissue. Samples were incubated with NCK1 monoclonal antibody (Product # MA5-34616), and followed by hematoxylin.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of NCK1 in Jurkat cells (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Samples were incubated with NCK1 monoclonal antibody (Product # MA5-34616) at a dilution of 1:50, followed by Alexa Fluor 488-conjugated goat anti rabbit IgG.