Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- MAB7008 - Provider product page
- Provider
- R&D Systems
- Product name
- Human NCK1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human NCK1 in direct ELISAs.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P16333
- Isotype
- IgG
- Antibody clone number
- 714506
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Phosphoproteomics of collagen receptor networks reveals SHP-2 phosphorylation downstream of wild-type DDR2 and its lung cancer mutants.
Iwai LK, Payne LS, Luczynski MT, Chang F, Xu H, Clinton RW, Paul A, Esposito EA, Gridley S, Leitinger B, Naegle KM, Huang PH
The Biochemical journal 2013 Sep 15;454(3):501-13
The Biochemical journal 2013 Sep 15;454(3):501-13
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human, Mouse, and Rat NCK1 by Western Blot. Western blot shows lysates of A172 human glioblastoma cell line, MOLT-4 human acute lymphoblastic leukemia cell line, BaF3 mouse pro-B cell line, and Y3-Ag rat myeloid cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human NCK1 Monoclonal Antibody (Catalog # MAB7008) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for NCK1 at approximately 47 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- NCK1 in MCF-7 Human Cell Line. NCK1 was detected in immersion fixed MCF-7 human breast cancer cell line using Mouse Anti-Human NCK1 Monoclonal Antibody (Catalog # MAB7008) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.