Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Other assay [1]
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Validation data
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- Product number
- 815304 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#815304, RRID:AB_2650661
- Product name
- Go-ChIP-Grade™ Purified anti-HDAC3
- Antibody type
- Monoclonal
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- 3G6
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- 2°C-8°C
- Handling
- Ambient RT
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Supportive validation
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- BioLegend (provider)
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- Experimental details
- Chromatin Immunoprecipitation (ChIP) was performed using commercial Protein-G coated 96 well high-throughput ChIP assay kit by loading 3 µg of cross-linked chromatin samples from HeLa cells with either A) 1:200 dilution of Go-ChIP-Grade™ Purified anti-HDAC3 Antibody (clone 3G6), B) equal amount of Purified Mouse IgG2a, κ Isotype Control Antibody (clone MG2a-53), or C) competitor’s ChIP-grade Purified anti-HDAC3 Antibody and D) equal amount of matched Isotype Control Antibody as recommended by the manufacturer. The enriched DNA was purified and quantified by real-time qPCR using primers targeting human EGR-1 gene region. EGR-1 (early growth response protein 1) is an early gene with diverse functions, including regulation of growth, differentiation and tumor suppression activity. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the 5% of total amount of input chromatin.
- Conjugate
- Unconjugated