815303

antibody from BioLegend

Targeting: HDAC3 HD3, KDAC3, RPD3, RPD3-2

Immunohistochemistry (Data presented on provider website)

Chromatin Immunoprecipitation (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

815303

Provider

BioLegend

Proper citation

BioLegend Cat#815303, RRID:AB_2650675

Product name

Go-ChIP-Grade™ Purified anti-HDAC3

Antibody type

Monoclonal

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antibody clone number

3G6

Vial size

25 µg

Concentration

0.5 mg/ml

Storage

2°C-8°C

Handling

Ambient RT

Other assay

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Chromatin Immunoprecipitation (ChIP) was performed using commercial Protein-G coated 96 well high-throughput ChIP assay kit by loading 3 µg of cross-linked chromatin samples from HeLa cells with either A) 1:200 dilution of Go-ChIP-Grade™ Purified anti-HDAC3 Antibody (clone 3G6), B) equal amount of Purified Mouse IgG2a, κ Isotype Control Antibody (clone MG2a-53), or C) competitor’s ChIP-grade Purified anti-HDAC3 Antibody and D) equal amount of matched Isotype Control Antibody as recommended by the manufacturer. The enriched DNA was purified and quantified by real-time qPCR using primers targeting human EGR-1 gene region. EGR-1 (early growth response protein 1) is an early gene with diverse functions, including regulation of growth, differentiation and tumor suppression activity. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the 5% of total amount of input chromatin.

Conjugate

Unconjugated