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LearnPA5-85378
antibody from Invitrogen Antibodies
Targeting: HDAC3
HD3, KDAC3, RPD3, RPD3-2
Western blot
Immunocytochemistry Immunoprecipitation Immunohistochemistry Chromatin Immunoprecipitation Other assayAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Chromatin Immunoprecipitation [1]
- Other assay [2]
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- Product number
- PA5-85378 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HDAC3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Keep as concentrated solution. Predicted reactivity: Mouse (99%), Rat (99%), Zebrafish (89%), Pig (100%), Chicken (95%), Rhesus Monkey (100%), Bovine (100%). Positive Control: 293T, A431, HeLa, HepG2, U87-MG, SK-N-SH, IMR32, SK-N-AS, Neuro2A, C8D30, NIH-3T3, Raw264.7, C2C12, PC-12, Rat2, NB2a. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.32 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HDAC3 Polyclonal Antibody detects HDAC3 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HDAC3 protein stained by HDAC3 Polyclonal Antibody (Product # PA5-85378) diluted at 1:1,000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin Polyclonal Antibody [GT114] (Product # MA5-31466) diluted at 1:1,000. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of HDAC3 in paraffin-embedded human ovarian cancer tissue using HDAC3 polyclonal antibody (Product # PA5-85378) at a dilution of 1:250.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Cross-linked ChIP was performed with MCF-7 chromatin extract and 5 µg of either control rabbit IgG or HDAC3 Polyclonal Antibody (Product # PA5-85378). The precipitated DNA was detected by PCR with primer set targeting to TFF1 or HK1.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of HDAC3 in 293T whole cell lysate, A) 293T whole cell lysate, B) control of preimmune rabbit IgG, C) immunoprecipitation of HDAC3 protein by of HDAC3 antibody with HDAC3 polyclonal antibody (Product # PA5-85378) using A) 40 µg, B and C) 2 µg of sample at a dilution of 1:1000. Sample was then incubated with anti-rabbit IgG secondary antibody. Prior to incubation with primary antibody, the sample was separated on 7.5% SDS-PAGE.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HDAC3 Polyclonal Antibody immunoprecipitates HDAC3 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A. 40 µg 293T whole cell lysate/extract B. Control with 2 µg of preimmune rabbit IgG C. Immunoprecipitation of HDAC3 protein by 2 µg of HDAC3 Polyclonal Antibody (Product # PA5-85378) 7.5% SDS-PAGE The immunoprecipitated HDAC3 protein was detected by HDAC3 Polyclonal Antibody (Product # PA5-85378) diluted at 1:1,000.
