NB100-1669
antibody from Novus Biologicals
Targeting: HDAC3
HD3, KDAC3, RPD3, RPD3-2
Antibody data
- Antibody Data
- Antigen structure
- References [7]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
Reference
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- Product number
- NB100-1669 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-1669, RRID:AB_2118721
- Product name
- Rabbit Polyclonal HDAC3 Antibody
- Antibody type
- Polyclonal
- Description
- Protein G purified. In HeLa, a 50 kDa band is observed.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Immunohistochemical Characterization of Histone Deacetylase as a Potential Prognostic Marker and Therapeutic Target in Endometrial Stromal Sarcoma.
Differential effect of HDAC3 on cytoplasmic and nuclear huntingtin aggregates.
HDAC4 promotes growth of colon cancer cells via repression of p21.
A sequential treatment of depsipeptide followed by 5-azacytidine enhances Gadd45beta expression in hepatocellular carcinoma cells.
Histone deacetylase 3 (HDAC3) and other class I HDACs regulate colon cell maturation and p21 expression and are deregulated in human colon cancer.
Direct p53 transcriptional repression: in vivo analysis of CCAAT-containing G2/M promoters.
Dynamic recruitment of NF-Y and histone acetyltransferases on cell-cycle promoters.
Baek MH, Park JY, Rhim CC, Park Y, Kim KR, Kim JH, Nam JH
Anticancer research 2016 May;36(5):2527-34
Anticancer research 2016 May;36(5):2527-34
Differential effect of HDAC3 on cytoplasmic and nuclear huntingtin aggregates.
Mano T, Suzuki T, Tsuji S, Iwata A
PloS one 2014;9(11):e111277
PloS one 2014;9(11):e111277
HDAC4 promotes growth of colon cancer cells via repression of p21.
Wilson AJ, Byun DS, Nasser S, Murray LB, Ayyanar K, Arango D, Figueroa M, Melnick A, Kao GD, Augenlicht LH, Mariadason JM
Molecular biology of the cell 2008 Oct;19(10):4062-75
Molecular biology of the cell 2008 Oct;19(10):4062-75
A sequential treatment of depsipeptide followed by 5-azacytidine enhances Gadd45beta expression in hepatocellular carcinoma cells.
Jiang C, Zhou B, Fan K, Heung E, Xue L, Liu X, Kirschbaum M, Yen Y
Anticancer research 2007 Nov-Dec;27(6B):3783-9
Anticancer research 2007 Nov-Dec;27(6B):3783-9
Histone deacetylase 3 (HDAC3) and other class I HDACs regulate colon cell maturation and p21 expression and are deregulated in human colon cancer.
Wilson AJ, Byun DS, Popova N, Murray LB, L'Italien K, Sowa Y, Arango D, Velcich A, Augenlicht LH, Mariadason JM
The Journal of biological chemistry 2006 May 12;281(19):13548-58
The Journal of biological chemistry 2006 May 12;281(19):13548-58
Direct p53 transcriptional repression: in vivo analysis of CCAAT-containing G2/M promoters.
Imbriano C, Gurtner A, Cocchiarella F, Di Agostino S, Basile V, Gostissa M, Dobbelstein M, Del Sal G, Piaggio G, Mantovani R
Molecular and cellular biology 2005 May;25(9):3737-51
Molecular and cellular biology 2005 May;25(9):3737-51
Dynamic recruitment of NF-Y and histone acetyltransferases on cell-cycle promoters.
Caretti G, Salsi V, Vecchi C, Imbriano C, Mantovani R
The Journal of biological chemistry 2003 Aug 15;278(33):30435-40
The Journal of biological chemistry 2003 Aug 15;278(33):30435-40
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: HDAC3 Antibody [NB100-1669] - Lane view shows a specific band for HDAC3 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: HDAC3 Antibody [NB100-1669] - Analysis of HDAC3 in HeLa cell lysate with anti-HDAC3 this antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: HDAC3 Antibody [NB100-1669] - Analysis human colon cancer using 1:50 conc. of HDAC3 antibody on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Nuclear staining of HDAC3 was observed. Staining was performed by Histowiz.