20226-1-AP

antibody from Proteintech Group

Targeting: ARF1

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

20226-1-AP

Provider

Proteintech Group

Product name

ARF1 antibody

Antibody type

Polyclonal

Description

KD/KO validated ARF1 antibody (Cat. #20226-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse and has been validated for the following applications: IF, IHC, IP, WB, ELISA.

Reactivity

Human, Mouse

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

Arf1 GTPase Regulates Golgi-Dependent G2/M Transition and Spindle Organization in Oocyte Meiosis.

Zhang KH, Zou YJ, Shan MM, Pan ZN, Ju JQ, Liu JC, Ji YM, Sun SC
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2024 Jan;11(4):e2303009

---

ARF1 prevents aberrant type I interferon induction by regulating STING activation and recycling.

Hirschenberger M, Lepelley A, Rupp U, Klute S, Hunszinger V, Koepke L, Merold V, Didry-Barca B, Wondany F, Bergner T, Moreau T, Rodero MP, Rösler R, Wiese S, Volpi S, Gattorno M, Papa R, Lynch SA, Haug MG, Houge G, Wigby KM, Sprague J, Lenberg J, Read C, Walther P, Michaelis J, Kirchhoff F, de Oliveira Mann CC, Crow YJ, Sparrer KMJ
Nature communications 2023 Nov 1;14(1):6770

---

Myristic acid as a checkpoint to regulate STING-dependent autophagy and interferon responses by promoting N-myristoylation.

Jia M, Wang Y, Wang J, Qin D, Wang M, Chai L, Fu Y, Zhao C, Gao C, Jia J, Zhao W
Nature communications 2023 Feb 7;14(1):660

---

The protein kinase D1-mediated classical protein secretory pathway regulates the Ras oncogene-induced senescence response.

Su Y, Wang P, Shen H, Sun Z, Xu C, Li G, Tong T, Chen J
Journal of cell science 2018 Mar 16;131(6)

---

Enterovirus 71 protease 2Apro and 3Cpro differentially inhibit the cellular endoplasmic reticulum-associated degradation (ERAD) pathway via distinct mechanisms, and enterovirus 71 hijacks ERAD component p97 to promote its replication.

Wang T, Wang B, Huang H, Zhang C, Zhu Y, Pei B, Cheng C, Sun L, Wang J, Jin Q, Zhao Z
PLoS pathogens 2017 Oct;13(10):e1006674

---

Essential role for GABARAP autophagy proteins in interferon-inducible GTPase-mediated host defense.

Sasai M, Sakaguchi N, Ma JS, Nakamura S, Kawabata T, Bando H, Lee Y, Saitoh T, Akira S, Iwasaki A, Standley DM, Yoshimori T, Yamamoto M
Nature immunology 2017 Aug;18(8):899-910

---

In vivo monitoring of the recruitment and activation of AP-1 by Arf1.

Sauvageau E, McCormick PJ, Lefrancois S
Scientific reports 2017 Aug 2;7(1):7148

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 20226-1-AP(ARF1 antibody) at dilution of 1:1000

Sample type

cell line

Immunocytochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using ARF1 antibody 20226-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Sample type

cell line

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human brain using 20226-1-AP(ARF1 antibody) at dilution of 1:100 (under 10x lens)

Sample type

tissue

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human brain using 20226-1-AP(ARF1 antibody) at dilution of 1:100 (under 40x lens)

Sample type

tissue