NB300-505

antibody from Novus Biologicals

Targeting: ARF1

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

NB300-505

Provider

Novus Biologicals

Proper citation

Novus Cat#NB300-505, RRID:AB_10001088

Product name

Mouse Monoclonal ARF1 Antibody

Antibody type

Monoclonal

Description

Unpurified. Detects ADP-ribosylation factor1 (Arf 1), Arf 3, Arf 5 and Arf 6 and at about a ten-fold reduction with Arf 4.

Reactivity

Human, Mouse, Rat, Bovine, Canine

Host

Mouse

Isotype

IgG

Vial size

100uL

Storage

Store at -20C. Avoid freeze-thaw cycles.

References

Submitted references

DRAM1 deficiency affects the organization and function of the Golgi apparatus.

Wei M, Zhu Z, Wu J, Wang Y, Geng J, Qin ZH
Cellular signalling 2019 Nov;63:109375

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TRAPPC13 modulates autophagy and the response to Golgi stress.

Ramírez-Peinado S, Ignashkova TI, van Raam BJ, Baumann J, Sennott EL, Gendarme M, Lindemann RK, Starnbach MN, Reiling JH
Journal of cell science 2017 Jul 15;130(14):2251-2265

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A CREB3-ARF4 signalling pathway mediates the response to Golgi stress and susceptibility to pathogens.

Reiling JH, Olive AJ, Sanyal S, Carette JE, Brummelkamp TR, Ploegh HL, Starnbach MN, Sabatini DM
Nature cell biology 2013 Dec;15(12):1473-85

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PI4P promotes the recruitment of the GGA adaptor proteins to the trans-Golgi network and regulates their recognition of the ubiquitin sorting signal.

Wang J, Sun HQ, Macia E, Kirchhausen T, Watson H, Bonifacino JS, Yin HL
Molecular biology of the cell 2007 Jul;18(7):2646-55

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Association of brefeldin A-inhibited guanine nucleotide-exchange protein 2 (BIG2) with recycling endosomes during transferrin uptake.

Shen X, Xu KF, Fan Q, Pacheco-Rodriguez G, Moss J, Vaughan M
Proceedings of the National Academy of Sciences of the United States of America 2006 Feb 21;103(8):2635-40

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Poliovirus proteins induce membrane association of GTPase ADP-ribosylation factor.

Belov GA, Fogg MH, Ehrenfeld E
Journal of virology 2005 Jun;79(11):7207-16

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Inhibition of membrane tubule formation and trafficking by isotetrandrine, an antagonist of G-protein-regulated phospholipase A2 enzymes.

Chan D, Strang M, Judson B, Brown WJ
Molecular biology of the cell 2004 Apr;15(4):1871-80

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Islet cell autoantigen of 69 kDa is an arfaptin-related protein associated with the Golgi complex of insulinoma INS-1 cells.

Spitzenberger F, Pietropaolo S, Verkade P, Habermann B, Lacas-Gervais S, Mziaut H, Pietropaolo M, Solimena M
The Journal of biological chemistry 2003 Jul 11;278(28):26166-73

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Regulation of Golgi structure and function by ARF-like protein 1 (Arl1).

Lu L, Horstmann H, Ng C, Hong W
Journal of cell science 2001 Dec;114(Pt 24):4543-55

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Intracellular distribution of Arf proteins in mammalian cells. Arf6 is uniquely localized to the plasma membrane.

Cavenagh MM, Whitney JA, Carroll K, Zhang Cj, Boman AL, Rosenwald AG, Mellman I, Kahn RA
The Journal of biological chemistry 1996 Sep 6;271(36):21767-74

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Intracellular distribution of Arf proteins in mammalian cells. Arf6 is uniquely localized to the plasma membrane.

Cavenagh MM, Whitney JA, Carroll K, Zhang Cj, Boman AL, Rosenwald AG, Mellman I, Kahn RA
The Journal of biological chemistry 1996 Sep 6;271(36):21767-74

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Intravesicular acidification correlates with binding of ADP-ribosylation factor to microsomal membranes.

Zeuzem S, Feick P, Zimmermann P, Haase W, Kahn RA, Schulz I
Proceedings of the National Academy of Sciences of the United States of America 1992 Jul 15;89(14):6619-23

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Intravesicular acidification correlates with binding of ADP-ribosylation factor to microsomal membranes.

Zeuzem S, Feick P, Zimmermann P, Haase W, Kahn RA, Schulz I
Proceedings of the National Academy of Sciences of the United States of America 1992 Jul 15;89(14):6619-23

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ADP-ribosylation factor is a subunit of the coat of Golgi-derived COP-coated vesicles: a novel role for a GTP-binding protein.

Serafini T, Orci L, Amherdt M, Brunner M, Kahn RA, Rothman JE
Cell 1991 Oct 18;67(2):239-53

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ADP-ribosylation factor is a subunit of the coat of Golgi-derived COP-coated vesicles: a novel role for a GTP-binding protein.

Serafini T, Orci L, Amherdt M, Brunner M, Kahn RA, Rothman JE
Cell 1991 Oct 18;67(2):239-53

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: ARF1 Antibody (1D9) [NB300-505] - Analysis of canine heart extract.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: ARF1 Antibody (1D9) [NB300-505] - Both normal and cancer biopsies of deparaffinized Human colon carcinoma tissues.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: ARF1 Antibody (1D9) [NB300-505] - Both normal and cancer biopsies of deparaffinized Human tonsil tissues.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: ARF1 Antibody (1D9) [NB300-505] - Both normal and cancer biopsies of deparaffinized Human liver tissues.

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: ARF1 Antibody (1D9) [NB300-505] - Analysis of MCF-7 cells compared to an isotype control (blue).

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: ARF1 Antibody (1D9) [NB300-505] - Analysis of Hela cells compared to an isotype control (blue).

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: ARF1 Antibody (1D9) [NB300-505] - Analysis of 3T3 cells compared to an isotype control (blue).