Antibody data
Product number STJ71062 - Provider product page
Provider St John's Laboratory
Product name Anti-ARF1 antibody (C-Term) (STJ71062) Antibody type Polyclonal Description Goat polyclonal antibody anti-ARF1 (C-Term) is suitable for use in ELISA and Immunohistochemistry research applications. Reactivity Human, Mouse, Rat, Canine Host Goat Conjugate Unconjugated Antigen sequence EGLDWLSNQLRNQK
Epitope NA Isotype IgG Antibody clone number NA Vial size NA Concentration NA Storage Store at-20 on receipt and minimise freeze-thaw cycles. Handling NA
ARF1 protein structure - STJ71062 shown in red.
Supportive validation
Submitted by
St John's Laboratory (provider)
Main image
Experimental details
STJ71062 Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line) , permeabilized with 0. 5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Sample type
NA
Validation comment
NA
Primary Ab dilution
NA
Other comments
NA
Secondary Ab
NA
Secondary Ab dilution
NA
Protocol
NA
Appendix
NA
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Supportive validation
Submitted by
St John's Laboratory (provider)
Main image
Experimental details
STJ71062 (1µg/ml) staining of A431 (A) and (0. 3ug/ml) of HeLa (B) and MCF7 (C) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
Sample type
NA
Validation comment
NA
Primary Ab dilution
NA
Other comments
NA
Secondary Ab
NA
Secondary Ab dilution
NA
Protocol
NA
Appendix
NA
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Supportive validation
Submitted by
St John's Laboratory (provider)
Main image
Experimental details
STJ71062 (1µg/ml) staining of NIH3T3 (A) , KNRK (B) and MDCK (C) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
Sample type
NA
Validation comment
NA
Primary Ab dilution
NA
Other comments
NA
Secondary Ab
NA
Secondary Ab dilution
NA
Protocol
NA
Appendix
NA
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