Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Other assay [2]
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Validation data
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- Product number
- V3811 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- ARF1 Antibody / ADP-ribosylation factor 1
- Antibody type
- Monoclonal
- Description
- This highly specific ARF1 antibody is suitable for use in Western blot/Immunohistochemistry applications with human, mouse and rat samples.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- 1A9/5
- Vial size
- 20 ug (with BSA and sodium azide), 100 ug (with BSA and sodium azide), 100 ug (without BSA or sodium azide), 7 ml IHC only format (if applicable)
- Concentration
- 0.2 mg/ml, 1 mg/ml
- Storage
- Store the ARF1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human HEK293 and HepG2 cell lysate with ARF1 antibody (clone 1A9/5). Predicted molecular weight ~21 kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human HeLa, kidney and HepG2 cell lysate with ARF1 antibody (clone 1A9/5). Predicted molecular weight ~21 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human liver tissue with ARF1 antibody (clone 1A9/5). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- SDS-PAGE analysis of purified, BSA-free ARF1 antibody (clone 1A9/5) as confirmation of integrity and purity.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- SDS-PAGE analysis of purified, BSA-free ARF1 antibody (clone 1A9/5) as confirmation of integrity and purity.