Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- NBP1-31753 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-31753, RRID:AB_2290022
- Product name
- Rabbit Polyclonal ARF1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ARF1 Antibody [NBP1-31753] - Sample (50 ug of whole cell lysate) A: mouse brain 12% SDS PAGE; antibody diluted at 1:10000.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ARF1 Antibody [NBP1-31753] - Sample (30 ug of whole cell lysate) A: A431 B: H1299 12% SDS PAGE; antibody diluted at 1:1000.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ARF1 Antibody [NBP1-31753] - Wild-type (WT) and ARF1 knownout (KO) HeLa cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with ARF1 antibody. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ARF1 Antibody [NBP1-31753] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with ARF1 antibody diluted by 1:1000.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: ARF1 Antibody [NBP1-31753] - Analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ARF1 Antibody [NBP1-31753] - Paraffin-embedded mouse prostate. ARF1 antibody diluted at 1:500.