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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunohistochemistry [1]
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- Product number
- PA5-21606 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-ARF1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- PA5-21606 targets ARF1 in IHC (P) and WB applications and shows reactivity with Human samples. The PA5-21606 immunogen is synthetic peptide contain a sequence corresponding to a region within amino acids 50 and 104 (P84077) of ARF1.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/ml
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ARF1 using A) 30 µg Neuro2A whole cell lysate (B) 30 µg GL261 whole cell lysate (C) 30 µg C8D30 whole cell lysate (D) 30 µg NIH-3T3 whole cell lysate (E) 30 µg BCL-1 whole cell lysate (F) 30 µg Raw264.7 whole cell lysate and G) 30 µg C2C12 whole cell lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with an ARF1 polyclonal antibody (Product # PA5-21606) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ARF1 using A) 30 µg PC-12 whole cell lysate and B) 30 µg Rat2 whole cell lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with an ARF1 polyclonal antibody (Product # PA5-21606) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ARF1 using 30µg of HepG2 lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with an ARF1 polyclonal antibody (Product # PA5-21606) at a dilution of 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of ARF1 in paraffin-embedded SW480 xenograft using an ARF1 polyclonal antibody (Product # PA5-21606) at a dilution of 1:100.
