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LearnTA500813
antibody from OriGene
Targeting: HSD17B10
17b-HSD10, ABAD, CAMR, ERAB, HADH2, MHBD, MRPP2, MRXS10, SDR5C1
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
- Flow cytometry [1]
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Validation data
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- Product number
- TA500813 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA500813, RRID:AB_11125053
- Product name
- Anti-HSD17B10 mouse monoclonal antibody, clone OTI5E2 (formerly 5E2)
- Antibody type
- Monoclonal
- Description
- Anti-HSD17B10 mouse monoclonal antibody, clone OTI5E2 (formerly 5E2)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- HSD17B10
- Isotype
- IgG
- Antibody clone number
- OTI5E2
- Vial size
- 100 µl
- Concentration
- 0.88 mg/ml
No comments: Submit comment
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HSD17B10 (RC201734, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HSD17B10.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-HSD17B10 monoclonal antibody.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-HSD17B10 mouse monoclonal antibody (TA500813) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY HSD17B10(RC201734).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-HSD17B10 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500813, Dilution 1:50)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded liver tissue within the normal limits using anti-HSD17B10mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500813, Dilution 1:50)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of liver tissue using anti-HSD17B10mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500813, Dilution 1:50)
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric analysis of Hela cells, using anti-HSD17B10 antibody(TA500813),(Red) compared to a nonspecific negative control antibody(TA50011)(Blue).
- Validation comment
- FC
